The amino acid sequences of two 13kDa polypeptides and partial amino acid sequence of 30kDa polypeptide of complex I from bovine heart mitochondria: Possible location of iron-sulfur clusters.
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概要
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Mitochondrial NADH: ubiquinone oxidoreductase (complex I) is the most complicated system in the respiratory chain. It consists of many subunits, some of which hold iron-sulfur clusters, but structural information is still limited. The amino acid sequences of two 13 kDa polypeptides, 13 kDa-A and 13 kDa-B polypeptides, of ironsulfur protein fraction (IP) of bovine heart mitochondrial complex I were determined by a combination of protease digestion, Edman degradation, and carboxypeptidase digestion. The 13 kDa-A polypeptide was composed of 96 amino acids with a molecular weight of 10, 536. The 13 kDa-B polypeptide consisted of 114 amino acids and had an acetylated amino terminus. The molecular weight of this protein was calculated to be 13, 130 including the acetyl group. These proteins had no obvious sequence similarity to other known proteins. The partial amino acid sequence of 30 kDa-B polypeptide of IP was also determined to reveal a characteristic arrangement of cysteine residues that could be involved in iron-sulfur cluster formation.
- 社団法人 日本生化学会の論文
著者
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Masui Ryoji
Department Of Bioligy Graduate School Of Science Osaka University
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Wakabayashi Sadao
Department Of Biology Faculty Of Science Osaka University
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Matsubara Hiroshi
Deparlment Of Internal Medicine School Of Medicine Kyoto University
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Hatefi Youssef
Division of Biochemistry, Research Institute of Scripps Clinic
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