Structure and Properties of the Recombinant NADH-Cytochrome b_5 Reductase of Physarum polycephalum
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概要
- 論文の詳細を見る
A cDNA for NADH–cytochrome b5 reductase of Physarum polycephalum was cloned from a cDNA library, and the nucleotide sequence of the cDNA was determined (accession no. AB259870). The DNA of 943 base pairs contains 5′- and 3′-noncoding sequences, including a polyadenylation sequence, and a coding sequence of 843 base pairs. The amino acid sequence (281 residues) deduced from the nucleotide sequence was 25 residues shorter than those of vertebrate enzymes. Nevertheless, the recombinant Physarum enzyme showed enzyme activity comparable to that of the human enzyme. The recombinant Physarum enzyme showed a pH optimum of around 6.0, and apparent Km values of 2 μM and 14 μM for NADH and cytochrome b5 respectively. The purified recombinant enzyme showed a typical FAD-derived absorption peak of cytochrome b5 reductase at around 460 nm, with a shoulder at 480 nm. These results suggest that the Physarum enzyme plays an important role in the organism.
- 社団法人 日本農芸化学会の論文
- 2007-03-23
著者
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MINAMI Yoshiko
Department of Biochemistry, Faculty of Science, Okayama University of Science
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YUBISUI Toshitsugu
Department of Biology, Faculty of Science, Kochi University
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Yubisui Toshitsugu
Department Of Biochemistry Faculty Of Science Okayama University Of Science
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Minami Yoshiko
Department Of Biochemistry Faculty Of Science Okayama University Of Science
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YAMAMOTO Shin-ya
Department of Materials Science, Graduate School of Engineering, Osaka Prefecture University
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Minami Yoshiko
Dep. Of Biochemistry Fac. Of Sci. Okayama Univ. Of Sci.
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IKEGAMI Terumi
Department of Biochemistry, Faculty of Science, Okayama University of Science
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KAMEYAMA Eiji
Department of Biochemistry, Faculty of Science, Okayama University of Science
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Kameyama Eiji
Department Of Biochemistry Faculty Of Science Okayama University Of Science
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Ikegami Terumi
Department Of Biochemistry Faculty Of Science Okayama University Of Science
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Yamamoto Shin-ya
Department Of Biochemistry Faculty Of Science Okayama University Of Science
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Yubisui Toshitsugu
Dep. Of Biochemistry Fac. Of Sci. Okayama Univ. Of Sci.
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