Potent inhibitory effect of trans9, trans11 isomer of conjugated linoleic acid on the growth of human colon cancer cells
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概要
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This study compared the growth inhibitory effects of pure conjugated linoleic acid (CLA) isomers [cis(c)9,c11-CLA, c9,trans(t)11-CLA, t9,t11-CLA, and t10,c12-CLA] on human colon cancer cell lines (Caco-2, HT-29 and DLD-1). When Caco-2 cells were incubated up to 72 h with 200 μM, each isomer, even in the presence of 10% fetal bovine serum (FBS), cell proliferation was inhibited by all CLA isomers in a time-dependent manner. The strongest inhibitory effect was shown by t9,t11-CLA, followed by t10,c12-CLA, c9,c11-CLA and c9,t11-CLA, respectively. The strongest effect of t9,t11-CLA was also observed in other colon cancer cell lines (HT-29 and DLD-1). The order of the inhibitory effect of CLA isomer was confirmed in the presence of 1% FBS. CLA isomers supplemented in the culture medium were readily incorporated into the cellular lipids of Caco-2 and changed their fatty acid composition. The CLA contents in cellular lipids were 26.2±2.7% for t9,t11-CLA, 35.9±0.3% for c9,t11-CLA and 46.3±0.8% for t10,c12-CLA, respectively. DNA fragmentation was clearly recognized in Caco-2 cells treated with t9,t11-CLA. This apoptotic effect of t9,t11-CLA was dose- and time-dependent. DNA fragmentation was also induced by 9c,11t-CLA and t10,c12-CLA. However, fragmentation levels with both isomers were much lower than that with t9,t11-CLA. t9t11-CLA treatment of Caco-2 cells decreased Bcl-2 levels in association with apoptosis, whereas Bax levels remained unchanged. These results suggest that decreased expression of Bcl-2 by t9t11-CLA might increase the sensitivity of cells to lipid peroxidation and to programmed cell death, apoptosis.
- Elsevier Inc.の論文
著者
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KOHNO Hiroyuki
Department of Oncologic Pathology, Kanazawa Medical University
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Tanaka T
Faculty Of Fisheries Hokkaido University
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Tanaka Takuji
京都大学 農学研究科食品生物科学
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Kohno H
Department Of Oncologic Pathology Kanazawa Medical University
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HOSOKAWA Masashi
Faculty of Fisheries, Hokkaido University
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Hosokawa M
Faculty Of Fisheries Sciences Hokkaido University
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Tsukui Takayuki
Faculty Of Fisheries Sciences Hokkaido University
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Tanaka T
Endoscopic And Photodynamic Medicine Hamamatsu University School Of Medicine
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Beppu Fumiaki
Sunny Health Holdings Co. Ltd. Osaka Jpn
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Tada Toshiji
Analytical Research Laboratories Fujisawa Pharmaceutical Co. Ltd.
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Tanaka Tatsuo
Endoscopic And Photodynamic Medicine Hamamatsu University School Of Medicine
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Hosokawa Masashi
Laboratory Of Biofunctional Material Chemistry Division Of Marine Bioscience Craduate School Of Fish
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Miyashita Kazuo
Faculty Of Fisheries Sciences Hokkaido University
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Kohno Hiroyuki
Department Of Serology Medical Research Institute Kanazawa Medical University
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Kamibayashi Masato
Faculty Of Fisheries Hokkaido University
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