Characterization of the α-helix region in domain 3 of the haemolytic lectin CEL-III: implications for self-oligomerization and haemolytic processes.

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概要

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• CEL-III is a haemolytic lectin, which has two beta-trefoil domains (domains 1 and 2) and a beta-sheet-rich domain (domain 3). In domain 3 (residues 284-432), there is a hydrophobic region containing two alpha-helices (H8 and H9, residues 317-357) and a loop between them, in which alternate hydrophobic residues, especially Val residues, are present. To elucidate the role of the alpha-helix region in the haemolytic process, peptides corresponding to different parts of this region were synthesized and characterized. The peptides containing the sequence that corresponded to the loop and second alpha-helix (H9) showed the strongest antibacterial activity for Staphylococcus aureus and Bacillus subtilis through a marked permeabilization of the bacterial cell membrane. The recombinant glutathione S-transferase (GST)-fusion proteins containing domain 3 or the alpha-helix region peptide formed self-oligomers, whereas mutations in the alternate Val residues in the alpha-helix region lead to decreased oligomerization ability of the fusion proteins. These results suggest that the alpha-helix region, particularly its alternate Val residues are important for oligomerization of CEL-III in target cell membranes, which is also required for a subsequent haemolytic action.

• The Japanese Biochemical Society,日本生化学会の論文
• 2008-01-01

著者

• HATAKEYAMA Tomomitsu

  Department of Applied Chemistry, Faculty of Engineering, Nagasaki University

• TSUDA Nobuaki

  Department of Applied Chemistry, Faculty of Engineering, Nagasaki University

• Tsuda Nobuaki

  Department Of Applied Chemistry Faculty Of Engineering Nagasaki University

• HISAMATSU Keigo

  Department of Applied Chemistry, Faculty of Engineering, Nagasaki University

• GODA Shuichiro

  Department of Applied Chemistry, Faculty of Engineering, Nagasaki University

• Hatakeyama Tomomitsu

  Dept Of Appl. Chem. Fac. Of Eng. Nagasaki Univ.

• Hatakeyama Tomomatsu

  Nagasaki University

• Hatakeyama Tomomitsu

  Department Of Agricultural Chemistry Faculty Of Agriculture Kyushu University

• Goda Shuichiro

  Dept of Appl. Chem., Fac. of Eng., Nagasaki Univ.

• Goda Shuichiro

  Nagasaki University

• Goda Shuichiro

  Dept Of Appl. Chem. Fac. Of Eng. Nagasaki Univ.

• Hisamatsu Keigo

  Department Of Applied Chemistry Faculty Of Engineering Nagasaki University

• Goda Shuichiro

  Department Of Applied Chemistry Faculty Of Engineering Nagasaki University

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