Characterization of the Interaction of Hemolytic Lectin CEL-III from the Marine Invertebrate, Cucumaria echinata, with Artificial Lipid Membranes: Involvement of Neutral Sphingoglycolipids in the Pore-Forming Process.
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概要
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The hemolytic lectin, CEL-III, is a Ca2+-dependent, galactose/N-acetylgalactosaminespecific lectin purified from the marine invertebrate, Cucumaria echinata (Holothuroidea). After binding to specific carbohydrates on the erythrocyte surface, CEL-III forms ion-permeable pores by oligomerizing in the membrane, which leads to colloid osmotic rupture of the cells. When incubated with liposomes composed of total lipids from the human erythrocyte membrane, GEL-III efficiently induced the leakage of carboxyfluorescein (CF) trapped in the vesicles, suggesting the presence of its receptor in the membrane lipids. The rate of CF-leakage increased with increasing temperature, although the hemolytic activity of CEL-III had been found to be much higher at lower temperatures (around 10°C). Identification of the receptor for GEL-III was performed by examining the ability of individual lipids from human erythrocytes to induce CF-leakage from DOPC-liposomes. As a result, the most effective receptor was found to be lactosyl ceramide (LacCer), while globoside (Gb4Cer) also showed slight induction of CF-leakage. On the other hand, abinding assay involving CEL-III-horseradish peroxidase conjugate indicated that CEL-III exhibits similar affinity for LacCer and Gb4Cer, suggesting that the structure or length of the carbohydrate portion of sphingoglycolipids is also relevant as to their ability to induce CF-leakage in addition to their affinity. Electron micrographs of CEL-III-treated liposomes revealed that CEL-III induced considerable morphological changes in the vesicles, while a clearly distinguishable oligomeric structure of the protein was not observed.
- 社団法人 日本生化学会の論文
著者
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KUWAHARA Hiromiki
Department of Applied Chemistry, Faculty of Engineering, Nagasaki University
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Hatakeyama Tomomitsu
Department Of Agricultural Chemistry Faculty Of Agriculture Kyushu University
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Aoyagi Haruhiko
Department Of Applied Chemistry Faculty Of Engineering Nagasaki University
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Sato Taiji
Department Of Applied Chemistry Faculty Of Engineering Nagasaki University
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Taira Etsuyo
Department Of Applied Chemistry Faculty Of Engineering Nagasaki University
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Niidome Takuro
Department of Applied Chemistry and Biochemistry, Graduate School of Science and Technology
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