Effect of Ca^<2+> on Refolding of the Recombinant Hemolytic Lectin CEL-III
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概要
- 論文の詳細を見る
CEL-III is a hemolytic lectin isolated from Cucumaria echinata. Although recombinant CEL-III (rCEL-III) expressed in Escherichia coli showed very weak hemolytic activity compared with native protein, it was considerably enhanced by refolding in the presence of Ca2+. This suggests that Ca2+ supported correct folding of the carbohydrate-binding domains of rCEL-III, leading to effective binding to the cell surface and subsequent self-oligomerization.
- 2009-05-23
著者
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Unno Hideaki
Department Of Applied Chemistry Faculty Of Engineering Nagasaki University
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Hatakeyama Tomomitsu
Dept Of Appl. Chem. Fac. Of Eng. Nagasaki Univ.
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Hatakeyama Tomomatsu
Nagasaki University
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Hatakeyama Tomomitsu
Department Of Agricultural Chemistry Faculty Of Agriculture Kyushu University
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Goda Shuichiro
Dept of Appl. Chem., Fac. of Eng., Nagasaki Univ.
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Goda Shuichiro
Nagasaki University
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Hisamatsu Keigo
Nagasaki University
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Goda Shuichiro
Dept Of Appl. Chem. Fac. Of Eng. Nagasaki Univ.
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Hisamatsu Keigo
Department Of Applied Chemistry Faculty Of Engineering Nagasaki University
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Goda Shuichiro
Department Of Applied Chemistry Faculty Of Engineering Nagasaki University
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