Effect of Salt Concentration on the pK_a of Acidic Residues in Lysozyme

スポンサーリンク

概要

• 論文の詳細を見る
• 1995-11-01

著者

• Ueda T

  Graduate School Of Pharmaceutical Sciences Kyushu University

• ABE Yoshito

  Graduate School of Pharmaceutical Sciences, Kyushu University

• IMOTO Taiji

  Faculty of Biotechnology and Life Science, Sojo University

• UEDA Tadashi

  Graduate School of Pharmaceutical Sciences, Kyushu University

• MOTOSHIMA Hiroyuki

  Graduate School of Pharmaceutical Sciences, Kyushu University

• IMOTO Taiji

  Graduate School of Pharmaceutical Sciences, Kyushu University

• HASHIMOTO Yoshio

  Graduate School of Pharmaceutical Sciences, Kyushu University

• IWASHITA Hiroki

  Graduate School of Pharmaceutical Sciences, Kyushu University

• TANAKA Yoshitugu

  Graduate School of Pharmaceutical Sciences, Kyushu University

• Abe Y

  Graduate School Of Pharmaceutical Sciences Kyushu University

• Abe Y

  Kyushu Univ. Fukuoka Jpn

• Motoshima H

  Saga Univ. Saga

• Motoshima Hiroyuki

  Department Of Metabolic Medicine Graduate School Of Medical Sciences Kumamoto University

• Motoshima Hiroyuki

  Department Of Metabolic Medicine Kumamoto University School Of Medicine

• Tanaka Y

  Nmr Section Graduate School Of Pharmaceutical Sciences Kyushu University

• Imoto T

  Faculty Of Biotechnology And Life Science Sojo University

• Imoto Taiji

  Graduate School Of Pharmaceutical Sciences

• Imoto Taiji

  Graduate School Of Pharmaceutical Science Kyushu University

• Iwashita Hiroki

  Graduate School Of Pharmaceutical Sciences Kyushu University

• 上田 卓也

  東京大学大学院新領域創成科学研究科メディカルゲノム専攻

• Ueda Tadashi

  Graduate School Of Pharmaceutical Sciences

• Imoto Taiji

  九州大学 薬学研究院

• Hashimoto Y

  Graduate School Of Pharmaceutical Sciences Kyushu University

• Tanaka Yuki

  Department Of Applied Science For Electronics And Materials Graduate School Of Engineering Sciences

• Abe Yoshito

  Graduate School Of Pharmaceutical Sciences Kyushu Univ

• Abe Y

  Department Of Structural Biology Biomolecular Engineering Research Institute

• Hashimoto Yoshio

  Graduate School Of Pharmaceutical Sciences Kyushu University

関連論文

• 哺乳類ミトコンドリアにおける新規翻訳因子の発見
• Coordination of Divalent Metal Cation to Amide Group to Form Adduct Ion in FAB Mass Spectrometry: Implication of Zn^ in Enzymatic Hydrolysis of Amide Bond
• 蛋白質解析装置 無細胞蛋白質合成系:大腸菌由来完全再構成系PURESYSTEM (バイオ高性能機器・新技術利用マニュアル) -- (各種解析装置の原理と使用例)
• 無細胞タンパク質合成系へのピュアアプローチ
• Technology 無細胞タンパク質合成系の新展開
• 翻訳に必須な因子の精製と再構成による生体外タンパク質合成系(Purified System)の構築
• CCA末端修復酵素の反応機構の解析
• Crystal Structures of K33 Mutant Hen Lysozymes with Enhanced Activities
• 3P-017 変性条件下における抗体λ6鎖の残存構造がアミロイド線維形成に及ぼす影響(蛋白質・物性(3),第46回日本生物物理学会年会)
• Analysis of Internal Motions of RNase T1 Complexed with a Productive Substrate Involving ^N NMR Relaxation Measurements
• Characterization of Refolded Hen Lysozyme Variant Lacking Two Outside Disulfide Bonds
• Fluctuations in Free or Substrate-Complexed Lysozyme and a Mutant of It Detected on X-Ray Crystallography and Comparison with Those Detected on NMR
• Thermostability of Doubly Glycosylated Recombinant Lysozyme
• Expression and Characterization of Human Rheumatoid Factor Single-Chain FV
• Construction of the Single-Chain Fv from 196-14 Antibody toward Ovarian Cancer-Associated Antigen CA125
• Detection of a Local Interaction of Hen Lysozyme under Highly Denaturing Conditions Using Chemically ^C-Enriched Methionine Resonance
• An Improved Method for Preparing Lysozyme with Chemically ^C-Enriched methionine Residues Using 2-Aminothiophenol as a Reagent of Thiolysis
• Analysis of the Stability of Mutant Lysozymes at Position 15 Using X-Ray Crystallography
• Influence of Mutations of the N-Cap Residue, Gly4, on Stability and Structure of Hen Lysozyme
• Analysis of the Stabilization of Hen Lysozyme by Helix Macrodipole and Charged Side Chain Interaction
• Situation of Monomethoxypolyethylene Glycol Covalently Attached to Lysozyme
• Analysis of the Transition State in the Unfolding of Hen Lysozyme by Introduction of Gly-Pro and Pro-Gly Sequences at the Same Site
• Stabilization of Lysozyme by Introducing N-Glycosylation Signal Sequence
• A Mutation Study of Catalytic Residue Asp 52 in Hen Egg Lysozyme^1
• Correlation between the Differences in the Free Energy Change and conformational Energy in the Folded State of Hen Lysozymes with Gly-Pro and Pro-Gly Sequences Introduced to the Same Site
• Effect of Salt Concentration on the pK_a of Acidic Residues in Lysozyme
• EFFECT OF CYP2C POLYMORPHISMS ON THE PHARMACOKINETICS OF PHENYTOIN IN JAPANESE PATIENTS WITH EPILEPSY
• Population Pharmacokinetics of Phenytoin in Japanese Patients with Epilepsy : Analysis with a Dose-Dependent Clearance Model
• Effect of Sucrose on Formation of the β-Amyloid Fibrils and D-Aspartic Acids in Aβ1-42
• Aggregation and Chemical Reaction in Hen Lysozyme Caused by Heating at pH 6 Are Depressed by Osmolytes, Sucrose and Trehalose^1
• Resolution and Characterization of Tryptophyl Fluorescence of Hen Egg-White Lysozyme by Quenching-and Time-Resolved Spectroscopy
• Functional and Structural Effects of Mutagenic Replacement of Asn37 at Subsite F on the Lysozyme-catalyzed Reaction
• Amino Acid Residues in Subsites E and F Responsible for the Characteristic Enzymatic Activity of Duck Egg-White Lysozyme
• Stabilization of Lysozyme against Irreversible Inactivation by Suppression of Chemical Reactions
• Effects of Additives on Irreversible Inactivation of Lysozyme at Neutral pH and 100℃
• Effect of Buffer Species on the Unfolding and the Aggregation of Humanized IgG
• A Method for the Detection of Asparagine Deamidation and Aspartate Isomerization of Proteins by MALDI/TOF-Mass Spectrometry Using Endoproteinase Asp-N
• ヌクレアーゼ型コリシンE4とE5の作用機構の研究
• コリシンE4とE5の持つヌクレアーゼ活性に関する研究 : 微生物
• リボソームに対するコリシンE3の作用 : 活性変異体を用いた再検討 : 微生物
• Analyses of Native Disulfide Bond Formations in the Early Stage of the Folding Process in Mutant Lysozymes Where the Long-Range Interactions in the Denatured State Were Modulated
• 3TA5-06 電子線結晶解析によって明らかになったヒト赤血球膜蛋白質バンド3膜貫通ドメインの立体構造(膜蛋白質,第47回日本生物物理学会年会)
• 再構築型無細胞タンパク質合成系 PURE system の開発と利用
• Enhancing Effect of Tumor Promoters, Phorbol Esters and Teleocidins on Nuclear Receptor-Mediated Transcription
• Identification of Protein Disulfide Isomerase as a Phorbol Ester-Binding Protein
• Pyridine-Promoted Factor-and Energy-Free Peptide Synthesis Systems Prepared from Various Organisms Including Prokaryote, Eukaryote, and Mitochondria
• Factor- and Energy-Free Peptide Synthesis Promoted by Aromatic Tertiary Amines Including Nucleic Acid-Related Compoiunds
• Template-Dependent Polypeptide Synthesis in a Factor- and Energy-Free Translation System Promoted by Pyridine
• 線形動物ミトコンドリアにおけるtRNAとEF-Tuの相互作用
• α-Glucosidase Inhibitors with a 4, 5, 6, 7-Tetrachlorophthalimide Skeleton Pendanted with a Cycloalkyl or Dicarba-closo-dodecaborane Group
• α-Glucosidase Inhibitors with a Phthalimide Skeleton : Stucture-Activity Relationship Study
• Modulators of Tumor Necrosis Factor α Production Bearing Dicarba-closo-dodecaborane as a Hydrophobic Pharmacophore
• プライマーとしてtRNA^/DNAを用いたHIV-1逆転写/テンプレート交換反応の効率
• ESI/Iontrap MS を用いた微量修飾ヌクレオシドの解析
• 牛ミトコンドアtRNA中に見いだされた未同定修飾塩基の構造解析
• LC/ESI MSを用いた修飾ウリジンの高感度, 特異的検出法の確立
• Genomic Clones Encoding Two Isoforms of Pokeweed Antiviral Protein in Seeds (PAP-S1 and S2) and the N-Glycosidase Activities of Their Recombinant Proteins on Ribosomes and DNA in Comparison with Other Isoforms^1
• 高品質なタンパク質を作るためのPURE Technology (特集 無細胞生命科学の創成)
• 無細胞遺伝子発現システムの現状と展望
• 蛋白質合成系の完全再構成--蛋白質誕生から成熟までのピュアアプローチ (細胞における 蛋白質の一生--生成・成熟・輸送・管理・分解・病態) -- (蛋白質の生成と成熟)
• Expression and Characterization of Bovine Mitochondrial Methionyl-tRNA Transformylase
• 多義語コドンの実験的検証
• ウシミトコンドリア蛋白質合成系におけるセリルtRNA合成酵素のクローニング
• リボソームRNAの触媒機能
• リボソームとRNAワールド rRNAの触媒機能はどこまでわかってきたか
• Possible Involvement of Atypiclal Protein Kinase C (PKC) in Glucose-Sensitive Expression of the Human Insulin Gene: DNA-Binding Activity and Transcriptional Activity of Pancreatic and Duodenal Homeobox Gene-1 (PDX-1) Are Enhanced via Calphostin C-Sensitiv
• A Case of Hyperinsulinemia of Undetermined Origin, Successfully Treated with Long-acting Octreotide
• 線形動物EF-Tu相同蛋白質の大腸菌における発現
• Characterization of the N-Oligosaccharides Attached to the Atypical Asn-X-Cys Sequence of Recombinant Human Epidermal Growth Factor Receptor
• Assignment of Imino Proton Signals of G-C Base Pairs and Magnesium Ion Binding: An NMR Study of Bovine Mitochondrial tRNA^_ Lacking the Entrire D Arm^1
• Purification and Phosphorylation of a M_r 25,000 Protein, an Effective Phosphate Acceptor for Casein Kinase II and Protein Kinase C,Detected in the Cytosolic Fraction of Xenopus laevis Oocytes
• A Novel Screening System for Self-mRNA Targeting Proteins
• Hairpin Structure of an RNA 28-mer, Which Contains a Sequence of the Enzyme Component of a Hammerhead Ribozyme System: Evidence for Tandem G: A Pairs That Are Not of Side-by-Side Type^1
• Properties of Hepatitis Delta Virus Ribozyme, Which Consists of Three RNA Oligomer Strands^1
• Phosphory lated Sites of M_r 25,000 Protein, a Putative Protein Phosphatase 2A Modulator, and Phosphorylation of the Synthetic Peptide Containing These Sites by Protein Kinase C^1
• Massspectrometric Analyses of Transmembrane Proteins in Human Erythrocyte Membrane
• 芳香族三級アミンを用いた新規な生体外タンパク質合成系とその翻訳系の起源研究への意義
• 多義語コドンの発見--複数のアミノ酸と結合するファジ-なtRNA
• リボソーム不活性化酵素や抗生物質を用いたピリジン蛋白質合成系の解析
• 大腸菌23SリボソームRNAによるペプチド結合形成反応
• ピリジン存在下の蛋白質合成系におけるリボソーム構成要素の解析
• Insect Lysozyme from House Fly (Musca domestica) Larvae: Possible Digestive Function Based on Sequence and Enzymatic Properties
• ウシミトコンドリアの翻訳開始機構におけるMet-tRNA transformylase(MTF)
• 蛋白質合成系におけるリボソームRNAの機能
• タンパク質合成系を利用したアミノ酸の鋳型重合
• 生体外蛋白質合成系の研究・開発と利用 有機触媒を用いた新規合成系に期待
• 哺乳類ミトコンドリアにおける新規翻訳因子の発見
• Induction of Sarcophaga Central Nervous System Remodeling by 20-Hydroxyecdysone In Vitro
• Kinetic Measurement of the Interaction between a Lysozyme and Its Immobilized Substrate Analogue by Means of Surface Plasmon Resonance
• Analyses of Native Disulfide Bond Formations in the Early Stage of the Folding Process in Mutant Lysozymes Where the Long-Range Interactions in the Denatured State Were Modulated
• 無細胞合成系PURE systemを基盤とした応用と人工細胞の構築
• A Case of Ectopic Adrenocorticotropic Hormone-producing Pancreatic Neuroendocrine Tumor with Multiple Liver Metastases
• 1S4p06 東京大学iGEM学生チームiGEM2011プロジェクトの紹介(iGEMを通した合成生物学研究者の育成,ワークショップ)
• Construction of a Screening System for Selecting Lysozyme Mutants Unable to Form a Stable Structure from Random Mutants
• tRNAの翻訳機能と修飾塩基(蛋白質合成におけるRNAの機能) (核酸化学の新展開--新しい機能性分子を求めて) -- (核酸の機能)
• 大腸菌全タンパク質の凝集性とシャペロン効果の網羅的な解析
• Influence of Mutations of the N-Cap Residue, Gly4, on Stability and Structure of Hen Lysozyme.
• A Mutation Study of Catalytic Residue Asp 52 in Hen Egg Lysozyme.
• An Improved Method for Preparing Lysozyme with Chemically 13C-Enriched Methionine Residues Using 2-Aminothiophenol as a Reagent of Thiolysis.
• Detection of a Local Interaction of Hen Lysozyme under Highly Denaturing Conditions Using Chemically 13C-Enriched Methionine Resonance.

もっと見る 閉じる

スポンサーリンク