A Mutation Study of Catalytic Residue Asp 52 in Hen Egg Lysozyme.

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概要

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• We constructed a system for the expression and secretion of mature hen lysozyme by yeast using an intermediate "secretion-signal cassette" vector, pKP1700, containing the yeast invertase signal sequence and an expression vector, pAM82, for secretion and maturation of the enzyme. Using this system, mutants of hen lysozyme were produced and the catalytic mechanism in hen lysozyme was definitely confirmed. The hydrolytic activity of D52A as to substrate (NAG)6 at pH 5.0 was obviously decreased to one-four hundredth of that of the wild type. The acidic limb of the pH-activity profile observed for the wild-type was not observed for D52A, and the pKa of Glu 35 on the alkaline limb was seen for both enzymes. Moreover, no structural change was detected on X-ray analysis of D52A. Therefore, we confirmed that dissociated Asp 52 assists catalysis by producing an electrostatic field and by stabilizing the oxocarbonium ion intermediate in the dissociated form.

• 社団法人 日本生化学会の論文

著者

• MOTOSHIMA Hiroyuki

  Graduate School of Pharmaceutical Sciences, Kyushu University

• YAMADA Keiko

  Graduate School of Pharmaceutical Sciences, Kyushu University

• MIKI Takeyoshi

  Graduate School of Pharmaceutical Sciences, Kyushu University

• Yasukochi Takanori

  Graduate School Of Pharmaceutical Sciences Kyushu University

• Imoto Taiji

  Graduate School Of Pharmaceutical Science Kyushu University

• Omura Tadahiro

  Graduate School Of Pharmaceutical Sciences Kyushu University

• Ueda Tadashi

  Graduate School Of Pharmaceutical Sciences

• Yamada Hidenori

  Graduate School Of Natural Science And Technology Okayama University

• Hashimoto Yoshio

  Graduate School Of Pharmaceutical Sciences Kyushu University

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