Construction of a Screening System for Selecting Lysozyme Mutants Unable to Form a Stable Structure from Random Mutants
スポンサーリンク
概要
- 論文の詳細を見る
- Japanese Biochemical Societyの論文
- 1999-09-01
著者
-
HASHIMOTO Yoshio
Graduate School of Pharmaceutical Sciences, Kyushu University
-
Imoto Taiji
Graduate School Of Pharmaceutical Science Kyushu University
-
Hashimoto Yoshio
Graduate School Of Pharmaceutical Sciences Kyushu University
-
KUNICHIKA Kaori
Graduate School of Pharmaceutical Sciences, Kyushu University
-
Kunichika Kaori
Graduate School Of Pharmaceutical Sciences Kyushu University
関連論文
- Fluctuations in Free or Substrate-Complexed Lysozyme and a Mutant of It Detected on X-Ray Crystallography and Comparison with Those Detected on NMR
- Thermostability of Doubly Glycosylated Recombinant Lysozyme
- Expression and Characterization of Human Rheumatoid Factor Single-Chain FV
- Construction of the Single-Chain Fv from 196-14 Antibody toward Ovarian Cancer-Associated Antigen CA125
- Detection of a Local Interaction of Hen Lysozyme under Highly Denaturing Conditions Using Chemically ^C-Enriched Methionine Resonance
- An Improved Method for Preparing Lysozyme with Chemically ^C-Enriched methionine Residues Using 2-Aminothiophenol as a Reagent of Thiolysis
- Analysis of the Stability of Mutant Lysozymes at Position 15 Using X-Ray Crystallography
- Influence of Mutations of the N-Cap Residue, Gly4, on Stability and Structure of Hen Lysozyme
- Analysis of the Stabilization of Hen Lysozyme by Helix Macrodipole and Charged Side Chain Interaction
- Situation of Monomethoxypolyethylene Glycol Covalently Attached to Lysozyme
- Analysis of the Transition State in the Unfolding of Hen Lysozyme by Introduction of Gly-Pro and Pro-Gly Sequences at the Same Site
- Stabilization of Lysozyme by Introducing N-Glycosylation Signal Sequence
- A Mutation Study of Catalytic Residue Asp 52 in Hen Egg Lysozyme^1
- Correlation between the Differences in the Free Energy Change and conformational Energy in the Folded State of Hen Lysozymes with Gly-Pro and Pro-Gly Sequences Introduced to the Same Site
- Effect of Salt Concentration on the pK_a of Acidic Residues in Lysozyme
- Effect of Sucrose on Formation of the β-Amyloid Fibrils and D-Aspartic Acids in Aβ1-42
- Aggregation and Chemical Reaction in Hen Lysozyme Caused by Heating at pH 6 Are Depressed by Osmolytes, Sucrose and Trehalose^1
- Functional and Structural Effects of Mutagenic Replacement of Asn37 at Subsite F on the Lysozyme-catalyzed Reaction
- Amino Acid Residues in Subsites E and F Responsible for the Characteristic Enzymatic Activity of Duck Egg-White Lysozyme
- Stabilization of Lysozyme against Irreversible Inactivation by Suppression of Chemical Reactions
- Effects of Additives on Irreversible Inactivation of Lysozyme at Neutral pH and 100℃
- A Method for the Detection of Asparagine Deamidation and Aspartate Isomerization of Proteins by MALDI/TOF-Mass Spectrometry Using Endoproteinase Asp-N
- Insect Lysozyme from House Fly (Musca domestica) Larvae: Possible Digestive Function Based on Sequence and Enzymatic Properties
- Kinetic Measurement of the Interaction between a Lysozyme and Its Immobilized Substrate Analogue by Means of Surface Plasmon Resonance
- Construction of a Screening System for Selecting Lysozyme Mutants Unable to Form a Stable Structure from Random Mutants
- Influence of Mutations of the N-Cap Residue, Gly4, on Stability and Structure of Hen Lysozyme.
- A Mutation Study of Catalytic Residue Asp 52 in Hen Egg Lysozyme.
- An Improved Method for Preparing Lysozyme with Chemically 13C-Enriched Methionine Residues Using 2-Aminothiophenol as a Reagent of Thiolysis.
- Detection of a Local Interaction of Hen Lysozyme under Highly Denaturing Conditions Using Chemically 13C-Enriched Methionine Resonance.