A Thermostable Laccase from Streptomyces lavendulae REN-7 : Purification, Characterization, Nucleotide Sequence, and Expression(Biochemistry & Molecular Biology)
スポンサーリンク
概要
- 論文の詳細を見る
We found a polyphenoloxidase (PPO) in the cell extract of Streptomyces lavendulae REN-7. About 0.8 mg of purified PPO was obtained from 200 g of the mycelia with a yield of 9.0%. REN-7-PPO showed broad substrate specificity toward various aromatic compounds. Moreover, this enzyme was capable of oxidation of syringaldazine, which is a specific substrate for laccase. Interestingly, REN-7-PPO retained its original activity after 20min of incubation at even 70℃. The gene encoding the PPO was cloned. Four copper-binding sites characteristics of laccases were contained in the deduced amino acid sequence. We constructed a highlevel expression system of this gene in Escherichia coli. The properties of the recombinant enzyme were identical that of wild-type. In conclusion, this PPO is a thermostable laccase.
- 社団法人日本農芸化学会の論文
- 2003-10-23
著者
-
Inamori Yoshihiko
Department Of Microbiology Osaka University Of Pharmaceutical Sciences
-
TSUJIBO Hiroshi
Osaka University of Pharmaceutical Sciences
-
MIYAMOTO Katsushiro
Osaka University of Pharmaceutical Sciences
-
INAMORI Yoshihiko
Osaka University of Pharmaceutical Sciences
-
ITO Masaaki
Central Research Laboratory, Hitachi, Ltd.
-
TSUJIBO Hiroshi
Department of Microbiology, Osaka University of Pharmaceutical Sciences
-
SUZUKI Takashi
Central Laboratory, Rengo Co., Ltd.
-
ENDO Kohki
Central Laboratory, Rengo Co., Ltd.
-
Endo Kohki
Central Laboratory Rengo Co. Ltd.
-
Tsujibo H
Department Of Microbiology Osaka University Of Pharmaceutical Sciences
-
Miyamoto K
Central Research Laboratories Osaka University Of Pharmaceutical Sciences
-
Suzuki Takashi
Central Laboratory Rengo Co. Ltd.
-
Ito Masaaki
Central Laboratory Rengo Co. Ltd.
-
Inamori Yoshihiko
Osaka College Of Pharmacy
-
Miyamoto Katsushiro
Osaka Univ. Of Pharmaceutical Sciences
関連論文
- Synthesis and Determination of Chirality of Optically Active α-Cyclopropylalanine
- Comparison of Conformations of Tetrapeptide Diastereomers Containing α-Phenyl-α-(2-pyridyl)glycine
- Molecular Cloning of the Gene Encoding a Novel β-N-Acetylhexosaminidase from a Marine Bacterium, Alteromonas sp. Strain O-7, and Characterization of the Cloned Enzyme(Biochemistry & Molecular Biology)
- Molecular Cloning of the Gene Encoding an Outer-Membrane-Associated β-N-Acetylglucosaminidase Involved in Chitin Degradation System of Alteromonas sp. Strain O-7
- Aggregation Feature of Fluorine-Substituted Benzene Rings and Intermodular C-H…F Interaction: Crystal Structure Analyses of Mono- and Trifluoro-L-phenylalanines
- Isolation of immunosuppressive phospholipids from marine bacteria
- Production of Angiotensin-Converting Enzyme Inhibitors from Baker's Yeast Glyceraldehyde-3-phosphate Dehydrogenase
- Bacillus stearothermophilus Glyceraldehyde-3-phosphate Dehydrogenase as a Source of Angiotensin-converting Enzyme Inhibitors(Biological Chemistry)
- Cloning and Nucleotide Sequence of the Gene Encoding a Serine Proteinase Inhibitor Named Marinostatin from a Marine Bacterium, Alteromonas sp. Strain B-10-31
- Crystal and Molecular Structure of an (S)-(+)-Enantiomer of Modafinil, a Novel Wake-Promoting Agent
- Ring-Field Extreme Ultraviolet Exposure System Using Aspherical Mirrors
- The Mechanism of the Bactericidal Activity of Hinokitiol
- The Excellence of Aomori Hiba (Hinokiasunaro) in Its Use as Building Materials of Buddhist Temples and Shinto Shrines
- Biological Activity of β-Dolabrin, γ-Thujaplicin, and 4-Acetyltropolone, Hinokitiol-Related Compounds(Biochemistry/Molecular Biology)
- Molecular Characterization of an Intracellular β-N-Acetylglucosaminidase Involved in the Chitin Degradation System of Streptomyces thermoviolaceus OPC-520
- Biological Activity of α-Thujaplicin, the Isomer of Hinokitiol(Biochemistry/Molecular Biology)
- A Thermostable Laccase from Streptomyces lavendulae REN-7 : Purification, Characterization, Nucleotide Sequence, and Expression(Biochemistry & Molecular Biology)
- Biological Activity of Tropolone(Biochemistry/Molecular Biology)
- Biological Activity of 4-Acetyltropolone, the Minor Component of Thujopsis dolabrata SIEB. et ZUCC. hondai MAK.
- Cloning and Expression of an α-L-Arabinofuranosidase Gene (stxIV) from Streptomyces thermoviolaceus OPC-520, and Characterization of the Enzyme(Biochemistry & Molecular Biology)
- Isolation and Characterization of the Genes Encoding Two Metalloproteases (MprI and MprII) from a Marine Bacterium, Alteromonas sp. Strain O-7(Biochemistry & Molecular Biology)
- Cloning, Sequencing, and Expression of the Gene Encoding an Intracellular β-D-Xylosidase from Streptomyces thermoviolaceus OPC-520(Biochemistry & Molecular Biology)
- Biological Activity of α-Thujaplicin, the Minor Component of Thujopsis dolabrata SIEB.et ZUCC.var.hondai MAKINO
- Cytotoxicity of the Hinokitiol-Related Compounds, γ-Thujaplicin and β-Dolabrin
- Family 19 Chitinases from Streptomyces thermoviolaceus OPC-520 : Molecular Cloning and Characterization
- Antifungal Activity of Hinokitiol-Related Compounds on Wood-Rotting Fungi and Their Insecticidal Activities
- Phytogrowth-Inhibitory Activities of β-Dolabrin and γ-Thujaplicin, Hinokitiol-Related Compounds and Constituents of Thujopsis dolabrata Sieb. et Zucc. var hondai MAKINO
- Biological Activity of Alkyl 2-(Acylthio)benzoates
- Purification and Characterization of a Thermostable Chitinase from Streptomyces thermoviolaceus OPC-520 and Cloning of the Encoding Gene
- Enantioselective Synthesis of a Phenylalanine Library Containing Alkyl Groups on the Aromatic Moiety : Confirmation of Stereostructure by X-Ray Analysis
- Inhibitory Activity of Podophyllotoxin and Matairesinol-derivative Lignans on the Root Growth of Brassica campestris
- Purification and Determination of the Chemical Structure of the Tyrosinase Inhibitor Produced by Trichoderma viride Strain H1-7 from a Marine Environment(Microbiology)
- Inhibitory Activity of 5-Thio-D-glucose on Plant Growth
- BIOLOGICAL ACTIVITIES OF STLIBENE DERIVATIVES
- PHYSIOLOGICAL ACTIVITIES OF DEOXYPODOPHYLLOTOXIN
- THE MECHANISMS OF DELAYED INSECTICIDAL ACTION OF STREPTOTHRICIN ANTIBIOTICS
- Intermolecular Association Model of Antifungal Polyene Macrolide via Concerted =C-H…O Interactions : X-ray Crystal Structure Analysis and Ab Initio Molecular-Orbital Calculations of Chainin
- Difference between Enzymatic and Chemical N-methylations of Protoberberine-Type Alkaloid, Dependent on the Stereoisomer of (-)-N-methyl-7, 8, 13, 13a-tetrahydroberberinium Salt
- Photon-Stimulated Ion Desorption Measurement of Organosilicon Resist Reactions in Extreme Ultraviolet Lithography
- Studies on Inhibitory Activities of Fukiic Acid Esters on Germination, α-Amylase and Carboxypeptidase A
- Conformational Comparison of μ-Selective Endomorphin-2 with its C-Terminal Free Acid, Studied by ^1H-NMR Spectroscopy and X-Ray Structure Analysis
- Insecticidal and Antifungal Activities of Aminorhodanine Derivatives
- Biological Activity of Purpurogallin
- Antimicrobial Activity and Metalloprotease Inhibition of Hinokitiol-Related Compounds, the Constituents of Thujopsis dolabrata S. and Z. hondai MAK.
- Biological Activities of 3,3'-Dihydroxy-α,β-diethylstilbene and Its Isomer, Diethylstilbestrol(Biological)
- Synthesis and Determination of Chirality of Optically Active α-Cycloalkylalanines
- Inhibitory Activities of 2-Pyridinecarboxylic Acid Analogs on Phytogrowth and Enzymes
- Inhibitory Activities of Rhodanine Derivatives on Plant Growth
- Antifungal and Insecticidal Activities of Daphnodorins A, B and C(Biological)
- Inhibitory Behavior of Cyanidine and Methylene Blue for Filament Formation of Tau Microtubule-binding Domain
- Structural Studies of [2',6'-Dimethyl-L-tyrosine^1]Endomorphin-2 Analogues Containing Proline Mimics
- Synthesis of Methyl and Ethyl 3-Alkylthiazolidine-4(R)-carboxylates and Their Roots Inhibition of the Growth of Brassica campestris
- Root-growth Inhibition by DL-Homocysteine Thiolactone and Its Related Compounds
- Identification of Genes, desR and desA, Required for Utilization of Desferrioxamine B as a Xenosiderophore in Vibrio furnissii
- Identification of the Positions of Disulfide Bonds of Chitinase from a Marine Bacterium,Alteromonas sp. Strain O-7
- PHYSIOLOGICAL ACTIVITY OF STREPTOTHRICIN ANTIBIOTIC AND STRUCTURE-ACTIVITY RELATIONSHIP
- RELATIONSHIP BETWEEN CONFORMATION AND BIOLOGICAL ACTIVITY OF RACEMOMYCIN-RELATED COMPOUNDS
- Expression in Escherichia coli of a Gene Encoding a Thermostable Chitinase from Streptomyces thermoviolaceus OPC-520
- The Conversion of Deoxypodophyl-lotoxin to Epipodophyllotoxin in Resting Cell System of Penicillium F-0543(Biological Chemistry)
- Conversion of Deoxypodophyllotoxin to Podophyllotoxin-related Compounds by Microbes(Biological Chemistry)
- The Conversion of Deoxypodophyllotoxin to Epipodophyllotoxin by Penicillium F-0543(Biological Chemistry)
- PC54 MICROBIAL TRANSFORMATION OF ANTITUMOR SUBSTANCE, DEOXYPODOPHYLLOTOXIN
- Synthesis and Plant Growth-inhibitory Activity of Methyl 3-(Alkylthio)carbonylthiazolidine-dine-4(R)-carboxylates
- Inhibitory Activities of 3-Thiophenecarboxylic Acid and Related Compounds on Plant Growth
- Cloning and Sequence Analysis of a Protease-encoding Gene from the Marine Bacterium Alteromonas sp. Strain O-7
- Purification and Characterization of β-N-Acetylglucosaminidase from Alteromonas sp.Strain O-7
- Site-directed Mutagenesis of Chitinase from Alteromonas sp. Strain O-7
- PURIFICATION AND GENE CLONING OF CHITINASE PRODUCED BY A MARINE BACTERIUM BELONGING TO GENUS ALTEROMONAS
- Evaluation of the Waveguide Effect in Proximity X-Ray Lithography Using an Optical Trace Method
- Purification and Properties of Two Types of Chitinases Produced by an Alkalophilic Actimomycete
- PURIFICATION AND CHARACTERIZATION OF TWO TYPES OF ALKALINE SERINE PROTEASES PRODUCED BY AN ALKALOPHILIC ACTINOMYCETE
- Amino Acid Compositions and Partial Sequences of Xylanases from a New Subspecies, Nocardiopsis dassonvillei subsp. alba OPC-18(Microbiology & Fermentation Industry)
- Amino Acid Compositions and Partial Sequences of Two Types of Alkaline Serine Proteases from Nocardiopsis dassonvillei subsp. prasina OPC-210(Microbiology & Fermentation Industry)
- Catalytic Properties of an Organic Solvent-Resistant Tyrosinase from Streptomyces sp. REN-21 and Its High-Level Production in E. coli
- Importance of CH-π Interaction Between I308 and Y310 Residues in the Third Repeat of Microtubule Binding Domain for Tau Filament Formation
- An Organic Solvent Resistant Tyrosinase from Streptomyces sp. REN-21 : Purification and Characterization
- Image Simulation of Extreme Ultraviolet Lithography Optics: Effect of Multilayer Coatings
- Intracellular Accumulation of Phenazine Antibiotics Produced by an Alkalophilic Actinomycete. I. Taxonomy, Isolation and Identification of the Phenazine Antibiotics(Microbiology & Fermentation Industry)
- Synthesis and Conformational Analysis of Homo-oligopeptides of α,α-Dicyclopropylglycine
- Synthesis and Conformational Analysis of Dcp-Containing Homo-Oligopeptides
- CH...π interplay between Ile308 and Tyr310 residues in the third repeat of microtubule binding domain is indispensable for self-assembly of three- and four-repeat tau
- CH-π Interaction between I308 and Y310 Residues is Required for Self Assembly of Full Length Tau
- Structural Studies on C-Amidated Amino Acids and Peptides : Function of Amide Group in Molecular Association in Crystal Structures of Val-Gly-NH_2, Ser-Phe-NH_2, Gly-Tyr-NH_2 and Pro-Tyr-NH_2 Hydrochloride Salts
- Identification and Characterization of an Outer Membrane Receptor Gene in Acinetobacter baumannii Required for Utilization of Desferricoprogen, Rhodotorulic Acid, and Desferrioxamine B as Xenosiderophores
- Characterization of a Gene Encoding the Outer Membrane Receptor for Ferric Enterobactin in Aeromonas hydrophila ATCC 7966^T