Site-directed Mutagenesis of Chitinase from Alteromonas sp. Strain O-7

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概要

• 論文の詳細を見る

• Chitinase (Chi85) from Alteromonas sp. strain O-7 contains the two conserved regions common to microbial and plant chitinases. We did site-directed mutagenesis of Chi85 to investigate the effects of the conserved amino acid residues on chitinase activity. We suggest that Asp-290 and Glu-292 of Chi85 may be the essential amino acid residues for the cleavage of β-glycosidic linkage of chitin.

• 社団法人日本農芸化学会の論文
• 1993-08-23

著者

• TSUJIBO Hiroshi

  Osaka University of Pharmaceutical Sciences

• MIYAMOTO Katsushiro

  Osaka University of Pharmaceutical Sciences

• INAMORI Yoshihiko

  Osaka University of Pharmaceutical Sciences

• IMADA Chiaki

  Institute of Microbial Chemistry

• OKAMI Yoshiro

  Institute of Microbial Chemistry

• Orikoshi Hideyuki

  Osaka University Of Pharmaceutical Sciences

• Okami Y

  Institute Of Microbial Chemistry

• Imada C

  Graduate School Of Marine Science And Technology Tokyo University Of Marine Science And Technology

• Miyamoto K

  Central Research Laboratories Osaka University Of Pharmaceutical Sciences

• Inamori Yoshihiko

  Osaka College Of Pharmacy

• Miyamoto Katsushiro

  Osaka Univ. Of Pharmaceutical Sciences

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