Intergenus Protoplast Fusion between Streptomyces and Micromonospora with Reference to the Distribution of Parental Characteristics in the Fusants
スポンサーリンク
概要
- 論文の詳細を見る
The protoplasts of two strains of Micromonospora which were sensitive to kanamycin(KM^s)and utilized raffinose(Raf^+), and one strain of Streptomyces griseus which was resistant to KM(KM^r)and did not utilize raffinose(Raf^-), were prepared, mixed in the presence of polyethylene glycol(PEG)and incubated on regeneration agar plates. Recombinant colonies showing KM^r・Raf^+ were obtained at a frequency of 2×10^<-6>. Their recombinants displayed a significant exchange of taxonomic characteristics between the two genera, although the majority appeared similar to the parent Micromonospora in their morphology as well as growth at 40℃. Their patterns of utilization of carbohydrates, amino acids and diammonium hydrogenphosphate were different from those of the Micromonospora. Intermediate or novel types which differed from their parents in their tolerance to NaCl and sensitivity to aminoglycoside antibiotics were also observed. Out of the 31 fusants obtained, two showed antimicrobial activity against Bacillus subtilis PCI 219, without any activity against Escherichia coli K-12 or Candida albicans 3147. The active substance may be a newly formed antibiotic, different from streptomycin in S.griseus.
- 社団法人日本生物工学会の論文
- 1999-08-25
著者
-
IMADA Chiaki
Institute of Microbial Chemistry
-
OKAMI Yoshiro
Institute of Microbial Chemistry
-
Okanishi Masanori
Institute Of Microbial Chemistry
関連論文
- Isolation of immunosuppressive phospholipids from marine bacteria
- Streptomyces kasugaensis sp. nov. : A New Species of Genus Streptomyces
- Thienodolin, a New Plant Growth-regulating Substance Produced by a Streptomycete Strain : I. Taxonomy and Fermentation of the Producing Strain, and the Isolation and Characterization of Thienodolin
- Mechanism of Multiple Aminoglycoside Resistance of Kasugamycin-producing Streptomyces kasugaensis MB273: Involvement of Two Types of Acetyltransferases in Resistance to Astromicin Group Antibiotics
- Identification of the Positions of Disulfide Bonds of Chitinase from a Marine Bacterium,Alteromonas sp. Strain O-7
- Production of Selenohomocystine as an Antibiotic by a Marine Bacillus sp. No. 14 with Selenomethionine Resistance
- IC202A, a New Siderophore with Immunosuppressive Activity Produced by Streptoalloteichus sp. 1454-19 I. Taxonomy, Fermentation, Isolation and Biological Activity
- A novel marine Bacillus with multiple amino acid analog resistance and selenomethionine-dependent antibiotic productivity
- Fluostatins A and B, New Inhibitors of Dipeptidyl Peptidase III, Produced by Streptomyces sp. TA-3391 I. Taxonomy of Producing Strain, Production, Isoloation, Physico-chemical Properties and Biological Properties
- Cloning and Sequence Analysis of a Protease-encoding Gene from the Marine Bacterium Alteromonas sp. Strain O-7
- Purification and Characterization of β-N-Acetylglucosaminidase from Alteromonas sp.Strain O-7
- Site-directed Mutagenesis of Chitinase from Alteromonas sp. Strain O-7
- Amidenin, a New Plant Growth-regulating Substance Isolated from Amycolatopsis sp.
- Thienodolin, a New Plant Growth-regulating Substance Produced by a Streptomycete Strain : II. Structure of Thienodolin
- PURIFICATION AND GENE CLONING OF CHITINASE PRODUCED BY A MARINE BACTERIUM BELONGING TO GENUS ALTEROMONAS
- Intergenus Protoplast Fusion between Streptomyces and Micromonospora with Reference to the Distribution of Parental Characteristics in the Fusants
- Leupeptins Produced by the Marine Alteromonas sp. B-10-31
- The Structure of Ribocitrin and its Structure-activity Relationship in the Inhibition of Dextransucrase of Streptococcus mutans E49
- Isolation and Properties of Ribocitrin