Agrobacterium-mediated Transformation of Javanica Rice cv. Rojolele

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概要

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• The Agrobacterium-mediated transformation system was extended to a famous Javanica rice variety, Rojolele, that is cultivated in Indonesia now. Efficient callus induction from immature and mature seeds of Rojolele did not succeed by any previous method for any rice cultivar. In this study, the callus from mature seeds of Rojolele exhibited a compact and nodular appearance on C medium after the carbon source and medium pH was modified. Scutellum-derived calli from mature seeds were co-cultivated with Agrobacterium tumefaciens strains EHA101 or LBA4404 that carried plasmid pAFT14, which contained the genes for β-glucuronidase (gus) and hygromycin resistance (hpt). Finally, the transformation efficiency of Rojolele variety using A. tumefaciens strain EHA101 (pAFT14) was improved to about 23%, similar to that of the Japonica rice variety Nipponbare. The seed fertility of transgenic Rojolele was more than 90%. The copy number of the transgene varied from one to three copies in the T0 transgenic lines. Both the gus and the hpt genes were inherited and expressed in the progeny.

• 2004-06-23

著者

• ANZAI Hiroyuki

  Gene Research Center, Ibaraki University

• Anzai H

  Gene Research Center Ibaraki University

• Anzai Hiroyuki

  Gene Research Center Ibaraki University

• HOSAKA Takehiko

  Gene Research Center, Ibaraki University

• Rachmawati Diah

  Gene Research Center, Ibaraki University

• Inoue Eiichi

  College of Agriculture, Ibaraki University

• Hosaka Takehiko

  Gene Research Center Ibaraki University

• Inoue E

  College Of Agriculture Ibaraki University

• Rachmawati Diah

  Gene Research Center Ibaraki University:faculty Of Biology Gadjah Mada University

• Rachmawati Diah

  Gene Research Center Ibaraki University

• INOUE Eiichi

  Gene Research Center, Ibaraki University

関連論文

• N-Linked Glycan Structures of Human Lactoferrin Produced by Transgenic Rice
• Molecular cloning of a novel Gα subunit gene and a Gβ subunit gene from the gray mold fungus Botrytis cinerea
• Studies on callus induction, plant regeneration and transformation of Javanica rice cultivars
• Production and Characterization of Recombinant Human Lactoferrin in Transgenic Javanica Rice
• Agrobacterium-mediated Transformation of Javanica Rice cv. Rojolele
• Characterization and Overcoming of Temperature-Dependent Lethality Exhibited by Hybrid Embryos from the Cross Nicotiana suaveolens × N.sylvestris
• Characterization of two cytochrome P450 monooxygenase genes of the pyripyropene biosynthetic gene cluster from Penicillium coprobium

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