Studies on callus induction, plant regeneration and transformation of Javanica rice cultivars

スポンサーリンク

概要

• 論文の詳細を見る

• The purpose of this study was to develop a reproducible efficient procedure for the transformation of Javanica rice cultivars from Indonesia. Five rice cultivars cultivated in Indonesia now were examined for their capacity on the callus growth, plant regeneration and transformation. Their potential was affected by genotype and medium. Regarding the quality of callus, type I calli produced higher plant regeneration frequency than type II calli. So type I calli were inoculated with A. tumefaciens harboring binary plasmid pAFT14, which had a hygromycin resistance (hpt) gene and a gus gene. In this study, we examined two media for two steps in the tissue culture process for transformation, i.e. callus inducing and plant regeneration. The results show that C-modified medium was the suitable media for callus induction in the most Javanica rice cultivars. Agrobacterium-mediated transformation system has been extended to five Javanica rice cultivars. Among them, Rojolele consistently gave the best performance.

• 2006-12-01

著者

• Anzai Hiroyuki

  Gene Research Center Ibaraki University

• Rachmawati Diah

  Gene Research Center, Ibaraki University

• Rachmawati Diah

  Gene Research Center Ibaraki University

関連論文

• N-Linked Glycan Structures of Human Lactoferrin Produced by Transgenic Rice
• Studies on callus induction, plant regeneration and transformation of Javanica rice cultivars
• Production and Characterization of Recombinant Human Lactoferrin in Transgenic Javanica Rice
• Agrobacterium-mediated Transformation of Javanica Rice cv. Rojolele
• Characterization of two cytochrome P450 monooxygenase genes of the pyripyropene biosynthetic gene cluster from Penicillium coprobium

スポンサーリンク