Preparation of a Photoactivatable Fluorescent Derivative of Lactose and Its Application to Photoaffinity Labeling of a Conger Eel Lectin
スポンサーリンク
概要
- 論文の詳細を見る
A photoactivatable heterobifunctional fluorescent reagent, 1-azido-5-naphthalene sulfonyl (ANS) hydrazide, was synthesized and characterized. ANS-hydrazide reacted with lactose to form a photoactivatable hydrazone. The derivative (ANS-lactose) had the same binding affinity for a conger eel lectin as lactose judging from the hemagglutinating-inhibition assay with rabbit erythrocytes. ANS-lactose was used for photoaffinity labeling of a conger eel lectin. The photolabeled lectin was digested with chymotrypsin to isolate photolabeled peptides by reversed-phase HPLC by monitoring fluorescence. A major labeled peptide was located at positions 31-45 in the lectin by amino acid analysis and N-terminal sequencing. The identified segment was close to the highly conserved region throughout animalβ-galactoside-binding lectins.
- 社団法人日本農芸化学会の論文
- 1994-06-23
著者
-
YAMAUCHI Fumio
Departments of Nephrology and Metabolism; School of Medicine, Tokai University
-
MURAMOTO Koji
Department of Applied Biological Chemistry, Faculty of Agriculture, Tohoku University
-
KAMIYA HISAO
School of Fisheries Sciences, Kitasato University
-
Kamiya H
Kitasato Univ. Iwate Jpn
-
Kamiya Hisao
School Of Fisheries Sciences Kitasato University
-
Muramoto Koji
Department Of Applied Biological Chemistry Faculty Of Agriculture Tohoku University
-
Muramoto Koji
Department Biomolecular Science Tohoku University
-
Yamauchi Fumio
Department Of Agricultural Chemistry Faculty Of Agriculture Tohoku University
-
Yamauchi Fumio
Department Of Applied Biological Chemistry Faculty Of Agriculture Tohoku University
関連論文
- Wegener's Granulomatosis with Relapsed Bleeding of Gastric Ulcers : A Case Report
- Amino Acid Composition of Proteins in Myctophid Fishes in the Subarctic and Tropical Pacific Ocean
- Modulating effect of acorn barnacle C-type lectins on the crystallization of calcium carbonate
- Reconstruction of Ancestral Conger Eel Galectins Have Revealed Their Molecular Diversification Process by Accelerated Evolution
- Structure and possible function of N-glycans of an invertebrate C-type lectin from the acorn barnacle Megabalanus rosa
- Acid Hydrolysis of Protein in a Microcapillary Tube for the Recovery of Tryptophan
- Characterization of L-amino acid oxidase and antimicrobial activity of aplysianin A, a sea hare-derived antitumor-antimicrobial protein
- Isolation and characterization of L-rhamnose-binding lectins from chum salmon (Oncorhynchus keta) eggs
- Tissue-specific Expression of Rhamnose-binding Lectins in the Steelhead Trout (Oncorhynchus mykiss)(Biochemistry & Molecular Biology)
- Distribution and Molecular Evolution of Rhamnose-binding Lectins in Salmonidae : Isolation and Characterization of Two Lectins from White-spotted Charr (Salvelinus leucomaenis) Eggs
- Microstructure and Orientation Distribution of Aragonite Crystals in Nacreous Layer of Pearl Shells
- マアナゴガレクチンの適応進化
- Effects of culture conditions on the expression level of lectin in Microcystis aeruginosa(freshwater cyanobacterium)
- Occurrence of Multiple Lectins in the Hemolymph of Kuruma Prawn Penaeus japonicus
- Molecular Properties and Activity of a Carboxyl-Terminal Truncated Form of Xylanase 3 from Aeromonas caviae W-61
- Gene of LukF-PV-like Component of Panton-Valentine Leukocidin in Staphylococcus aureus P83 is Linked with lukM
- Substitution of Lysine for Arginine in the N-Terminal 217th Amino Acid Residue of the HγII of Staphylococcal γ-Hemolysin Lowers the Activity of the Toxin
- ANTITUMOR ACTIVITY OF AQUEOUS EXTRACTS OF MARIRE ANIMALS
- Rhamnose-binding Lectins from Steelhead Trout (Oncorhynchus mykiss) Eggs Recognize Bacterial Lipopolysaccharides and Lipoteichoic Acid
- High-level Expression and Characterization of Fully Active Recombinant Conger Eel Galectins in Eschericia coli(Biochemistry & Molecular Biology)
- Comparison of the amino acid sequences of acorn barnacle lectins showing different inhibitory activities toward the crystal growth of calcium carbonate
- A Novel Rhamnose-binding Lectin Family from Eggs of Steelhead Trout(Oncorhynchus mykiss) with Different Structures and Tissue Distribution
- Accelerated Evolution in the Protein-coding Region of Galectin cDNAs, Congerin I and Congerin II, from Skin Mucus of Conger Eel (Conger myriaster)
- Inheritance of "Group A Saponin" in Soybean Seed(Analytical Chemistry)
- Characterization of the Lectin from the Skin Mucus of the Kingklip Genypterus capensis
- Characterization of Lectins from the Skin Mucue of the Loach Misgurnus anguillicaudatus
- Structural Analyses on Aggregates of Graft Copolypeptides Containing Tryptophan in Aqueous Media and Efficiency of Drug Release
- Purification of a novel allergen (SAP-1), which induces allergic asthma, from the red soft coral (Alcyonium gracillimum)
- Purification and Characterization of the Lectins of the Soft Coral Lobophytum variatum
- Analysis of the Hemolymph Proteins from the Acorn Barnacle Megabalanus rosa by Two-dimensional Gel Electrophoresis
- Preparation of a Photoactivatable Fluorescent Derivative of Lactose and Its Application to Photoaffinity Labeling of a Conger Eel Lectin
- Preparation and Characterization of a Cleavable Photoactivable Heterobifunctional Fluorescent Reagent(Analytical Chemistry)
- Simple Method for Detecting Glycoproteins Dot-blotted or Electro-blotted on to a Polyvinylidene Difluoride Membrane
- STUDIES ON THE UTILIZATION OF GRAPE PART I. COMPOSITION OF GRAPE JUICES
- Diverse Sugar-Binding Specificities of Marine Invertebrate C-Type Lectins
- Diverse Sugar-Binding Specificities of Marine Invertebrate C-Type Lectins
- STUDIES ON THE PAPER CHROMATOGRAPHY OF GLUCOSE POLYMERS (I) Correlation of the Colour Reaction with the Chemical Structure (II) Correlation of the Rf Value with the Chemical Structure
- Effective Digestion of Casein with Agar Gel-Entrapped Cell Wall Fraction-Bound Enzymes of Aspergillus oryzae as Immobilized Proteases
- Partial Characterization of Aspergillus oryzae Cell Wall Fraction-Bound Enzyme Related to Immobilized Biocatalyst
- Enzyme Immobilization on Glycosylated Edible Proteins(Food & Nutrition)
- Enzyme Immobilization by the Formation of Enzyme Coating on Small Pore-size Ion-Exchangers(Biological Chemistry)
- Relationship between the Limited Proteolysis of Glycinin and Its Conformation(Food & Nutrition)
- Localization and Partial Characterization of Acid Proteinase Bound on Aspergillus oryzae Cell Wall Fraction on Media Compositions
- STUDIES ON SUGARS IN ACID AND ENZYME HYDROLYSATES OF POLYSACCHARIDES IV. ON THE β-LINKED SUGARS IN THE OLIGOSACCHARIDES FRACTION OF THE COMMERCIAL GLUCOSES PRODUCED BY ACID OR BY ENZYME HYDROLYSIS FROM SWEET POTATO STARCH
- STUDIES ON SUGARS IN ACID AND ENZYME HYDROLYSATES OF POLYSACCARIDES III. ON THE SUGAR COMPOSITION OF THE COMMERCIAL GLUCOSES PRODUCED BY ACID OR BY ENZYME HYDROLYSIS FROM SWEET POTATO STARCH
- Angiotensin I-Converting Enzyme Inhibitors in Autolysates of Squid Liver and Mantle Muscle
- Effects of Food Lectins on the Transport System of Human Intestinal Caco-2 Cell Monolayers
- Isolation and Electrophoretic Analysis of Heat-induced Products of Mixed Soybean 7S and 11S Globulins
- On the Role of Disulfide Bonds in Polymerization of Soybean 7S Globulin during Storage
- Effect of Acetylation on Hardening and Softening Properties of Soybean Protein-Water Suspending Systems
- Determination of Sulfhydryl and Disulfide Contents of Soybean 11S Globulin and Their Change by Lyophilization
- Spectroscopic Studies on the Aggregates and the Dissociates Induced by Heating Soybean 11S Globulin in the Presence of N-Ethylmaleimide
- Relationship between the Properties of Emulsion Systems Stabilized by Soy Protein Digests and the Protein Conformation
- Emulsifying Properties of Soybean β-Conglycinin and Glycinin: Evaluation by Turbidimetry
- Isolation and Characterization of a Proteolipid in Defatted Soybean Meals
- Comparison of the Multiple Agglutinins of the Acorn Barnacle, Megabalanus rosa
- Electrophoretical and Differential Thermal Analysis of Soybean 11S Globulin Heated in the Presence of N-Ethylmaleimide
- Isolation and Identification of a New Type of β-Conglycinin in Soybean Globulins
- Effects of Lipids on the Rheological Properties of Aqueous Soybean Protein Dispersions
- Analysis of the State of Aromatic Amino Acid Residues in Heated Soybean 7S Globulin by Absorption Derivative Spectrophotometry and Spectrofluorimetry
- Effect of Trimethylammonium Residue-containing Compounds on Gel Formation with Heating and Freezing of Soybean Protein
- Rheological Behavior of Soybean Protein-Lipid-Water Gel, from a Small Distortion to Rupture
- Effect of Alkalic Salts on Hardening and Softening Properties of Soybean Protein-Water Suspending Systems
- Rhamnose-binding lectins induce respiratory burst activity in macrophage cells from rainbow trout