Isolation and Identification of a New Type of β-Conglycinin in Soybean Globulins
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概要
- 論文の詳細を見る
A new type of β-conglycinin was found in precipitates of a crude glycinin fraction at pH 6.4 and low ionic strength. The protein was purified by ammonium sulfate fractionation, Con A-Sepharose 4B affinity chromatography and ion-exchange chromatography on DEAE-Sepharose CL-6B. The purity check and identification of the protein were performed by gel electrophoresis, gel filtration, N-terminal amino acid analysis, immunochemical analysis and ultra-centrifugal analysis. This protein (designated as B0-conglycinin) was composed of three identical subunits which were identical with the β-subunit in β-conglycinins at 0.5 ionic strength, but differed from the other β-conglycinins in that B0-conglycinin formed an insoluble aggregate at ionic strengths less than 0.2.
- 社団法人 日本農芸化学会の論文
著者
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Yamauchi Fumio
Department Of Agricultural Chemistry Faculty Of Agriculture Tohoku University
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Shibasaki Kazuo
Department Of Agricultural Chemistry Faculty Of Agriculture Tohoku University
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Sato Wataru
Department Of Environmental And Health Science Tohoku Pharmaceutical University
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Sato Masaaki
Department Of Anesthesiology Jichi Medical School
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KAMATA Yoshiro
Department of Food Chemistry, Faculty of Agriculture, Tohoku University
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