Diverse Sugar-Binding Specificities of Marine Invertebrate C-Type Lectins

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概要

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• The sugar-binding specificities of C-type lectins isolated from marine invertebrates were investigated by frontal affinity chromatography (FAC) using 100 oligosaccharides. The lectins included BRA-2 and BRA-3, multiple lectins from the hemolymph of the acorn barnacle, Megabalanus rosa, and BRL from the acorn barnacle, Balanus rostatus. The diverse sugar-binding specificities of the C-type lectins were determined by FAC analysis. BRA-2 recognized α2-6 sialylation but not α2-3 sialylation on glycans. On the other hand, BRA-3 showed high affinity for oligosaccharides with α-linked non-reducing terminal galactose, but not for sialylated forms, and BRL showed enhanced recognition activity towards Lewisx and Lewisa epitopes.

• 社団法人 日本農芸化学会の論文

著者

• MURAMOTO Koji

  Department of Applied Biological Chemistry, Faculty of Agriculture, Tohoku University

• JIMBO MITSURU

  School of Fisheries Sciences, Kitasato University

• KAMIYA HISAO

  School of Fisheries Sciences, Kitasato University

• OGAWA Tomohisa

  Department of Biomolecular Science, Graduate School of Life Sciences, Tohoku University

• HIRABAYASHI Jun

  Research Center for Glycoscience, National Institute of Advanced Industrial Science and Technology (

• MATSUBARA Hiroki

  Department of Chemistry, Faculty of Sciences, Kyushu University

• Matsubara Hiroki

  Graduate School Of Life Sciences Tohoku University

• NAKAMURA-TSURUTA Sachiko

  Research Center for Glycoscience, National Institute of Advanced Industrial Science and Technology

• Hirabayashi Jun

  Research Center For Glycoscience National Institute Of Advanced Industrial Science And Technology

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