Comparison of the Multiple Agglutinins of the Acorn Barnacle, Megabalanus rosa
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概要
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We investigated the subunit structures of the three agglutinins (BRA-1, BRA-2, and BRA-3) isolated from the coelomic fluid of the acorn barnacle, Megabalanus rosa, by high-speed gel filtration, gel electrophoresis, CD spectra, and peptide mapping. BRA-2 (Mr 140, 000) and BRA-3 (Mr 64, 000) were glycoproteins composed of subunits having two identical basic units cross-linked by disulfide bonds. The basic units of BRA-2 and BRA-3 had molecular weights of 22, 000 and 18, 000, respectively. They did not have a common structure. BRA-1 (Mr 330, 000) was composed of subunits homologous to that of BRA-2. The binding properties of these agglutinins against rabbit erythrocyte ghosts were also investigated after labeling the agglutinins with fluorescein isothiocyanate.
- 社団法人 日本農芸化学会の論文
著者
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Kamiya Hisao
School Of Fisheries Sciences Kitasato University
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Muramoto Koji
School Of Fisheries Sciences Kitasato University
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MURAMOTO Koji
School of Fisheries Sciences, Kitasato University
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OGATA Kyoko
School of Fisheries Sciences, Kitasato University
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