Effects of Food Lectins on the Transport System of Human Intestinal Caco-2 Cell Monolayers
スポンサーリンク
概要
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The effects of 16 lectins isolated from foodstuff on the transport system across human intestinal Caco-2 cell monolayers were investigated by using four fluorescent markers: lucifer yellow (LY) for the paracellular pathway, fluorescein (FL) for the monocarboxylic acid transporter-mediated pathway, rhodamine 123 for the P-glycoprotein-mediated efflux pathway, and calcein for the multidrug resistance associated protein-related efflux pathway. The transepithelial electrical resistance (TER) values for the monolayers were also measured. WGA from wheat germ, ABA from white mushroom, AOL from Aspergillus oryzae, and CSL3 from chum salmon eggs (each at 100 µg/mL) decreased the TER value by 20–40% which resulted in increased LY transport. These lectins, as well as such other lectins as SBA from soybean, RBA from rice bran, and Con A from jack bean, affected other transport pathways too. These results indicate that the lectins modulated the transepithelial transport system in different ways, probably because of their specific binding characteristics toward Caco-2 cell monolayers.
著者
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OGAWA Tomohisa
Department of Biomolecular Science, Graduate School of Life Sciences, Tohoku University
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Muramoto Koji
Department Biomolecular Science Tohoku University
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Yamamoto Shintaro
Department of Earth and Science, Graduate School of Science, Osaka University
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Naganuma Takako
Department Biomolecular Science Tohoku University
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MURAMOTO Koji
Department of Biomolecular Sciences, Graduate School of Life Sciences, Tohoku University
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NEMOTO Ryo
Department of Biomolecular Sciences, Graduate School of Life Sciences, Tohoku University
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TOMIYAMA Mai
Department of Biomolecular Sciences, Graduate School of Life Sciences, Tohoku University
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YAMAMOTO Shintaro
Department of Biomolecular Sciences, Graduate School of Life Sciences, Tohoku University
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