Treatment with a Histone Deacetylase Inhibitor After Nuclear Transfer Improves the Preimplantation Development of Cloned Bovine Embryos

スポンサーリンク

概要

• 論文の詳細を見る

• We examined the effects of treatment with histone deacetylase inhibitors (HDACi), trichostatin A (TSA) and scriptaid (SCR), on the blastocyst formation rate in bovine somatic cell nuclear transferred (SCNT) embryos derived from fibroblast cells. Three fibroblast cell lines (L1, L2 and L3) were used as somatic cell donors to produce SCNT embryos (L1, L2 and L3 embryos, respectively). In Experiment 1, we compared the in vitro developmental competence of L1 embryos treated with various concentrations of TSA for different time periods following chemical activation. Embryos treated with 5 nM TSA for 20 h showed a significantly increased blastocyst formation rate compared with untreated controls. In Experiment 2, we examined the effect of TSA (5 nM) treatment of L1, L2 and L3 embryos as well as the effect of treatment of L1, L2 and L3 embryos with various concentrations of SCR on in vitro developmental competence. It was found that 5 nM TSA treatment significantly increased the blastocyst formation rate in L1 and L3 embryos but did not have an influence on the development of L2 embryos. On the other hand, 5 nM SCR treatment significantly increased the blastocyst formation rates of L1 and L2 embryos compared with controls. However, there was no significant increase in the blastocyst formation rate of L3 embryos when they were treated with SCR. In Experiment 3, acetylation of H4K12 was examined in donor cells and pronuclear-stage L1, L2 and L3 embryos treated with 5 nM TSA or 5 nM SCR by immunostaining. The level of H4K12 acetylation was different among donor cells. The staining intensities in the TSA-treated L1 and L3 embryos and SCR-treated L2 embryos were significantly higher than those of untreated embryos. These results suggest that HDACi treatment of bovine SCNT embryos improves the blastocyst formation rate; however, the optimal treatment conditions may differ among donor cell lines.

• 日本繁殖生物学会の論文

著者

• Akagi Satoshi

  Reproductive Biology and Technology Research Team, National Institute of Livestock and Grassland Sci

• Matsukawa Kazutsugu

  Reproductive Biology and Technology Research Team, National Institute of Livestock and Grassland Sci

• TAKAHASHI Seiya

  Reproductive Biology and Technology Research Team, National Institute of Livestock and Grassland Sci

• Akagi Satoshi

  Department Of Animal Breeding And Reproduction National Institute Of Livestock And Grassland Science

• Matsukawa Kazutsugu

  Department Of Animal Breeding And Reproduction National Institute Of Livestock And Grassland Science

• MIZUTANI Eiji

  Reproductive Biology and Technology Research Team, National Institute of Livestock and Grassland Sci

• FUKUNARI Kazuhiro

  Iwate Prefecture Central Livestock Hygiene Service Center

• KANEDA Masahiro

  Reproductive Biology and Technology Research Team, National Institute of Livestock and Grassland Sci

• WATANABE Shinya

  Reproductive Biology and Technology Research Team, National Institute of Livestock and Grassland Sci

関連論文

• Culture of Bovine Embryos on a Polydimethylsiloxane (PDMS) Microwell Plate
• OJ-043 Inhibitory Effects of Simvastatin and Imatinib on PDGF-induced Proliferation and Migration of Smooth Muscle Cells Obtained from Patients with IPAH(OJ08,Pulmonary Circulation (H),Oral Presentation (Japanese),The 73rd Annual Scientific Meeting of The
• OE-110 High-dose Epoprostenol Reverses Pulmonary Hypertension by Pulmonary Artery Smooth Muscle Cell Apoptosis in Patients with Idiopathic Pulmonary Arterial Hypertension(OE19,Pulmonary Circulation (H),Oral Presentation (English),The 73rd Annual Scientifi
• インヒビンα-鎖ワクチンによる初期免疫および再免疫後の牛における卵巣反応とホルモン分泌
• OE-224 Pulmonary Arterial Augmentation Index Reflects Disease Severity in Patients with Pulmonary Arterial Hypertension(Pulmonary circulation(01)(H),Oral Presentation(English),The 72nd Annual Scientific Meeting of the Japanese Circulation Society)
• PJ-832 Epoprostenol Improves the Vasoreactivity to Oxygen in Patients with PAH(Pulmonary circulation-4, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
• OE-247 Acute Hemodynamic Effect of Bosentan in Patients with Idiopathic Pulmonary Arterial Hypertension Already Treated with Epoprostenol(Pulmonary circulation-1 (H) OE42,Oral Presentation (English),The 70th Anniversary Annual Scientific Meeting of the Ja
• Culture of Bovine Embryos on a Polydimethylsiloxane (PDMS) Microwell Plate
• PJ-830 Predictive Index for Long-Term Deterioration in the Patients with Pulmonary Arterial Hypertension(Pulmonary circulation-4, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
• Treatment with a Histone Deacetylase Inhibitor After Nuclear Transfer Improves the Preimplantation Development of Cloned Bovine Embryos
• In Vitro Development of Equine Oocytes from Preserved Ovaries after Intracytoplasmic Sperm Injection
• Caprine somatic cell nuclear transfer using in vivo matured oocytes collected by laparoscopic follicular aspiration
• Nuclear transfer using a bovine mammary epithelial cell line (BMEC)
• Treatment with a Histone Deacetylase Inhibitor after Nuclear Transfer Improves the Preimplantation Development of Cloned Bovine Embryos
• Evaluation of Equine Oocytes from Preserved Ovaries Using Intracytoplasmic Sperm Injection
• Developmental Ability of Somatic Cell Nuclear Transferred Embryos Aggregated at the 8-cell Stage or 16- to 32-cell Stage in Cattle
• Intermittent arm ischemia induces vasodilatation of the contralateral upper limb
• The Effect of Ovary Storage and In Vitro Maturation on mRNA Levels in Bovine Oocytes : A Possible Impact of Maternal ATP1A1 on Blastocyst Development in Slaughterhouse-derived Oocytes
• Constant transmission of mitochondrial DNA in intergeneric cloned embryos reconstructed from swamp buffalo fibroblasts and bovine ooplasm
• Influence of Intergeneric/Interspecies Mitochondrial Injection : Parthenogenetic Development of Bovine Oocytes after Injection of Mitochondria Derived from Somatic Cells
• Acute Vasoreactivity Testing With Nicardipine in Patients With Pulmonary Arterial Hypertension
• Downregulation of Histone Methyltransferase Genes SUV39H1 and SUV39H2 Increases Telomere Length in Embryonic Stem-like Cells and Embryonic Fibroblasts in Pigs
• Takotsubo cardiomyopathy associated with pulmonary resections after induction chemoradiotherapy for non-small cell lung cancer
• Acute Vasoreactivity Testing With Nicardipine in Patients With Pulmonary Arterial Hypertension
• Downregulation of Histone Methyltransferase Genes SUV39H1 and SUV39H2 Increases Telomere Length in Embryonic Stem-like Cells and Embryonic Fibroblasts in Pigs

もっと見る 閉じる

スポンサーリンク