インヒビンα-鎖ワクチンによる初期免疫および再免疫後の牛における卵巣反応とホルモン分泌

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To explore a strategy for superovulation in heifers, effects of immunization and re-immunization of Japanese beef heifers against inhibin on ovarian response and hormonal profiles were investigated. On day 9 of the estrous cycle (day 0=day of estrus), ten heifers were primary injected intramuscularly (i.m.) either with inhibin vaccine (recombinant ovine inhibin α-subunit in oil emulsion, 125 μg/ml) or a placebo (Montanide:Marcol adjutant alone) followed by two booster injections at one estrous cycle intervals (immunized group). One year later, 3 injections of inhibin vaccine were given the same as in the first year (re-immunized group). Inhibin antibody titers in plasma of immunized heifers increased after the second injection of inhibin vaccine and in 3 out of 5 immunized heifers and multiple-ovulation (2.4 ± 0.6, n=5) was induced after the third injection. On the other hand, in the re-immunized heifers, the plasma inhibin antibody titers increased sharply after the first injection of inhibin vaccine and in 4 out of 5 re-immunized heifers multiple ovulation (4.4 ± 1.1, n=5) was induced after the first injection of inhibin vaccine. In all re-immunized heifers multiple-ovulation was induced after the third injection of inhibin vaccine (5.0 ± 1.1, n=5). The results also showed that the ovulation rates were positively correlated to circulating inhibin antibody titers and plasma levels of FSH. These results confirmed that active immunization against inhibin α-subunit induced multiple ovulation mainly by attenuation of the suppressive effect of inhibin on FSH secretion in heifers and indicated that re-immunization against inhibin was a good way to induce multiple-ovulation in cattle.

インヒビンα-鎖ワクチンによる初期免疫および再免疫後の牛における卵巣反応とホルモン分泌

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