Developmental Ability of Somatic Cell Nuclear Transferred Embryos Aggregated at the 8-cell Stage or 16- to 32-cell Stage in Cattle
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概要
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Aggregation of somatic cell nuclear transfer (SCNT) embryos in mice is reported to improve full-term development. In the present study, we attempted to improve the development of SCNT embryos by aggregation in cattle. In Experiment 1, to examine the effect of the timing of aggregation on in vitro development of cumulus-cell NT embryos, we aggregated two or three SCNT embryos (2X or 3X embryos) at the 1-cell, 8-cell and 16- to 32-cell stages. Irrespective of the timing of aggregation, 3X embryos developed to the blastocyst stage at a high rate. However, aggregation did not improve the total blastocyst formation rate of the embryos used. The cell numbers of 3X embryos aggregated at the 1-cell stage and 2X embryos tended to be higher than that of single NT embryos (1X embryos). Furthermore, a significant increase in cell number was observed in 3X embryos aggregated at the 8-cell stage and 16- to 32-cell stage. In Experiment 2, we used fibroblast cells as nuclear donors and examined in vitro development of 3X embryos aggregated at the 8-cell stage and 16- to 32-cell stage. As a result, 3X embryos had high blastocyst formation rates and higher cell numbers than 1X embryos, which was consistent with the results of Experiment 1. In Experiment 3, we examined the full-term development ability of 3X embryos aggregated at the 8-cell stage and 16- to 32-cell stage. After transfer of fibroblast-derived NT embryos into recipient animals, a significantly higher pregnancy rate was obtained on Day 60 in 3X embryos than in 1X embryos. Two embryos aggregated at 8-cell stage and one embryo aggregated at the 16- to 32-cell stage developed to term, while no pregnancies derived from 1X embryos that lasted to Day 60. However, two of the cloned calves were stillborn. These results suggest that aggregation of the 8-cell stage or 16- to 32-cell stage SCNT embryos may improve the pregnancy rate, but that it cannot reduce the high incidence of fetal loss and stillbirth, which is often observed in bovine SCNT.
- 2011-08-01
著者
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Hosoe Misa
National Institute of Agrobiological Sciences
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Akagi Satoshi
Department Of Animal Breeding And Reproduction National Institute Of Livestock And Grassland Science
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Akagi Satoshi
Reproductive Biology And Technology Research Team National Institute Of Livestock And Grassland Scie
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Adachi Noritaka
Ibaraki Prefectural Livestock Research Center
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Takahashi Seiya
Reproductive Biology And Technology Research Team National Institute Of Livestock And Grassland Scie
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Takahashi Seiya
Department Of Animal Breeding And Reproduction National Institute Of Livestock And Grassland Science
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Matsukawa Kazutsugu
Reproductive Biology And Technology Research Team National Institute Of Livestock And Grassland Scie
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Matsukawa Kazutsugu
Department Of Animal Breeding And Reproduction National Institute Of Livestock And Grassland Science
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Mizutani Eiji
Lab. For Genomic Reprogramming Riken Center For Developmental Biology
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Mizutani Eiji
Reproductive Biology And Technology Research Team National Institute Of Livestock And Grassland Scie
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Kubo Masanori
National Institute Of Animal Health
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AKAGI Satoshi
Animal Breeding and Reproduction Division, National Institute of Livestock and Grassland Science
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YAMAGUCHI Daisuke
Ibaraki Prefectural North Livestock Hygiene Service Center
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MATSUKAWA Kazutsugu
Research and Education Faculty, Kochi University
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MIZUTANI Eiji
Animal Breeding and Reproduction Division, National Institute of Livestock and Grassland Science
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TAKAHASHI Seiya
Headquarters, National Agriculture and Food Research Organization
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Matsukawa Kazutsugu
Research And Education Faculty Kochi University
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