Influence of Intergeneric/Interspecies Mitochondrial Injection; Parthenogenetic Development of Bovine Oocytes after Injection of Mitochondria Derived from Somatic Cells
スポンサーリンク
概要
- 論文の詳細を見る
Interspecies/intergeneric mitochondrial heteroplasmy can occur in interspecies/intergeneric hybrid embryos or following nuclear transfer. In the present study, intergeneric buffalo (iBubalus bubalis/i) mitochondria (WB-mt) or interspecies murine (iMus spretus/i) mitochondria (M-mt) were injected into bovine (iBos taurus/i) oocytes, and the subsequent embryonic development was characterized. Fibroblast mitochondria (WB-mt or M-mt) were microinjected into iin vitro/i matured bovine oocytes followed by oocyte activation by a combination of electrical stimulation and 6-dimethylaminopurine treatment. After seven days of culture, embryo development was evaluated. The copy number of specific mtDNA populations (introduced and native mtDNA) from heteroplasmic oocytes was estimated using real-time PCR. The results illustrated that oocytes injected with either WB-mt or M-mt can develop to the blastocyst stage (20.6% and 19.6%). Cleavage division rates and development to the morula stage in oocytes injected with WB-mt were lower (76.2% and 45.9%, respectively) in comparison with uninjected oocytes (89.2% and 59.1%, respectively) (P0.05). However, no differences were found in comparing M-mt injected oocytes and controls (P0.05). An increase in bovine mtDNA copy number was observed at the expanded blastocyst stage of injected embryos (P0.01), while the number of injected mtDNA was stable throughout development. This study demonstrates that interspecies/intergeneric mitochondrial injected bovine oocytes have the ability to develop to the blastocyst stage after parthenogenetic activation and that injected mtDNA was neither selectively destroyed nor enhanced through development. Moreover, injected intergeneric mitochondria had a demonstrated influence on bovine parthenogenetic development and mtDNA replication.
著者
関連論文
- Culture of Bovine Embryos on a Polydimethylsiloxane (PDMS) Microwell Plate
- In Vitro Development of Equine Oocytes from Preserved Ovaries after Intracytoplasmic Sperm Injection
- Treatment with a Histone Deacetylase Inhibitor after Nuclear Transfer Improves the Preimplantation Development of Cloned Bovine Embryos
- Evaluation of Equine Oocytes from Preserved Ovaries Using Intracytoplasmic Sperm Injection
- Developmental Ability of Somatic Cell Nuclear Transferred Embryos Aggregated at the 8-cell Stage or 16- to 32-cell Stage in Cattle
- Constant transmission of mitochondrial DNA in intergeneric cloned embryos reconstructed from swamp buffalo fibroblasts and bovine ooplasm
- Influence of Intergeneric/Interspecies Mitochondrial Injection : Parthenogenetic Development of Bovine Oocytes after Injection of Mitochondria Derived from Somatic Cells
- Influence of Intergeneric/Interspecies Mitochondrial Injection; Parthenogenetic Development of Bovine Oocytes after Injection of Mitochondria Derived from Somatic Cells