Immunohistochemical demonstration of chromogranin A in endocrine organs of the rat and horse by use of region-specific antibodies
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概要
- 論文の詳細を見る
Chromogranin A (CgA) is an acidic glycoprotein that is co-stored with hormones or neurotransmitters in granular components of endocrine cells and neurons, and released together with them in response to adequate stimulation. In addition to acting as a packaging protein, CgA functions as a precursor molecule that yields several bioactive peptides by proteolytic cleavage. The purpose of this study is to elucidate how different the processing of CgA is among endocrine tissues by immunostaining using multiple region-specific antisera, and to evaluate the availability of region-specific antisera. When various endocrine organs of rats were immunostained with four region-specific antisera against rat CgA (CgA 1-28, 94-130, 296-314, and 359-389), all amine/peptide-secreting endocrine tissues except the pineal body were stained positively. The adrenal medulla and gastric endocrine cells were equally intensely immunoreactive to all four antisera, while the other endocrine tissues, represented by pancreatic islets, showed different staining patterns depending on the antiserum. These results suggest that the processing of CgA differs from tissue to tissue. An antiserum against horse CgA 335-365, corresponding to rat CgA 359-389 which shows the highest concentration in the plasma and urine of the rat, again stained all endocrine tissues of the horse except the pineal body. Therefore, the anti-horse CgA 335-365 serum is useful for immunohistochemical survey of horse CgA, and may make possible the establishment of a CgA assay system for the measurement of CgA in the plasma, urine and saliva.
- 北海道大学の論文
- 2001-05-31
著者
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SATO Fumio
Laboratory of Racing Chemistry
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IWANAGA Toshihiko
Laboratory of Histology and Cytology, Hokkaido University Graduate School of Medicine
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YANAIHARA Noboru
Yanaihara Institute Inc.
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SATO Fumio
Department of Astronomy and Earth Sciences, Tokyo Gakugei University
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Iwanaga Toshihiko
Laboratory Of Anatomy Department Of Biomedical Sciences Graduate School Of Veterinary Medicine Hokka
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Iwanaga Toshihiko
Laboratory Of Histology And Cytology Department Of Functional Morphology Graduate School Of Medicine
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Sato F
Equine Research Institute
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HASHIMOTO Yoshiharu
Laboratory of Anatomy, Department of Biomedical Sciences, Graduate School of Veterinary Medicine, Ho
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OHKI Hiroyuki
Laboratory of Anatomy, Department of Biomedical Sciences, Graduate School of Veterinary Medicine, Ho
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SATO Fumio
NHK Science and Technical Research Laboratories
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Sato F
Japan Racing Association Utsunomiya Jpn
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Yanaihara N
Yanaihara Inst. Shizuoka Jpn
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Iwanaga T
Hokkaido Univ. Sapporo
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Sato F
Nhk Science And Technical Research Laboratories
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Iwanaga T
Laboratory Of Histology And Cytology Hokkaido University Graduate School Of Medicine
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Iwanaga Toshihiko
Laboratories Of Anatomy Graduate School Of Veterinary Medicine Hokkaido University
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Ohki Hiroyuki
Laboratory Of Anatomy Department Of Biomedical Sciences Graduate School Of Veterinary Medicine Hokka
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Hashimoto Yoshiharu
Laboratory Of Anatomy Department Of Biomedical Science Graduate School Of Veterinary Medicine Hokkai
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Yanaihara Noboru
Yanaihara Institute
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SATO Fumio
Laboratory of Molecular and Cellular Biology, Equine Research Institute, Japan Racing Association
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