Development of a Method for the Identification of S-IgA-Coated Bacterial Composition in Mouse and Human Feces
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概要
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Some commensal intestinal bacteria in humans and mice are coated with secretory immunoglobulin A (S-IgA). It has been suggested that the S-IgA coating of commensal bacteria does not occur at random and thus identification of S-IgA-coated bacterial genera/species should help in elucidating the interaction between S-IgA and commensal intestinal bacteria, but no method of identifying the genera/species of S-IgA-coated bacteria has been established. To identify S-IgA-coated bacterial composition, we developed a method combining immunohistochemical detection of S-IgA and subsequent 16S rRNA targeted fluorescence in situ hybridization (FISH) analysis. Human and mouse fecal S-IgA coated bacterial composition was evaluated by this newly developed method with 10 frequently-used FISH probes. Fecal S-IgA-coated bacterial composition was successfully analyzed by this method, and this suggests that Enterobacteriaceae is preferably coated with S-IgA, whereas Bacteroides/Prevotella and Lactobacillus/Enterococcus groups appear to be poorly coated with S-IgA.
- 社団法人 日本農芸化学会の論文
著者
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HARA HIROSHI
Laboratory of Nutritional Biochemistry, Graduate School of Agriculture, Hokkaido University
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IWANAGA Toshihiko
Laboratory of Histology and Cytology, Hokkaido University Graduate School of Medicine
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Inoue Ryo
Laboratory of Animal Science, Kyoto Prefectural University
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Tsuruta Takeshi
Laboratory of Nutritional Biochemistry, Department of Applied Bioscience, Graduate School of Agricul
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YAJIMA Takaji
Meiji Dairies Research Chair, Creative Research Initiative Sousei, Hokkaido University
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