Improved Isolation Methods for Mucosal Leukocytes from Small and Large Intestines in Rats
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概要
- 論文の詳細を見る
We optimized the isolation protocol for intraepithelial lymphocytes (IELs) and lamina propria lymphocytes (LPLs) from the rat small intestine, and LPLs from even the rat large intestine. The major population of IELs in the small intestine was considered to be from the villus epithelia. The cytotoxicity of mucosal leukocytes was comparable among isolated fractions from both the small and large intestines, regardless of the population differences. Further analyses of the cells collected from other lymphoid tissues demonstrated that CD161+ cells selectively accumulated in the intestinal lamina propria and did not recirculate through the lymph ducts. Our modified isolation protocol enables the collection of mucosal immune cells from the rat intestines without any deterioration of cell function and could contribute to a better understanding of dietary influences on the mucosal immune system.
- 社団法人 日本農芸化学会の論文
- 2009-08-23
著者
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HARA HIROSHI
Laboratory of Nutritional Biochemistry, Graduate School of Agriculture, Hokkaido University
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Hara H
Lab. Of Nutritional Biochemistry Div. Of Applied Bioscience Graduate School Of Agriculture Hokkaido
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Tsukahara Takamitsu
Kyoto Institute of Nutrition and Pathology
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Hara Hiroshi
Toxicology Laboratory Chugai Pharmaceutical Co. Ltd.
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YAMADA Kaori
Kyoto Institute of Nutrition & Pathology
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NAKAYAMA Keizo
Kyoto Institute of Nutrition and Pathology, Inc.
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Nakayama Keizo
Kyoto Institute Of Nutrition And Pathology
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Yoo Yung-choon
Department Of Microbiology College Of Medicine Konyang University
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Lee Jin-ha
Research Faculty Of Agriculture Hokkaido University
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Yamada Kaori
Kyoto Institute Of Nutrition And Pathology
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LEE Jae-Sung
Laboratory of Nutritional Biochemistry, Division of Applied Bioscience, Research Faculty of Agricult
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OKA Kohsuke
Laboratory of Nutritional Biochemistry, Division of Applied Bioscience, Research Faculty of Agricult
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OBARA Mie
Laboratory of Nutritional Biochemistry, Division of Applied Bioscience, Research Faculty of Agricult
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NISHIMUKAI Megumi
Laboratory of Nutritional Biochemistry, Division of Applied Bioscience, Research Faculty of Agricult
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ISHIZUKA Satoshi
Laboratory of Nutritional Biochemistry, Division of Applied Bioscience, Research Faculty of Agricult
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Obara Mie
Laboratory Of Nutritional Biochemistry Division Of Applied Bioscience Research Faculty Of Agricultur
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Nishimukai Megumi
Laboratory Of Nutritional Biochemistry Division Of Applied Bioscience Research Faculty Of Agricultur
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Hara Hiroshi
Laboratory Of Nutritional Biochemistry Division Of Applied Bioscience Graduate School Of Agriculture
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Tsukahara Takamitsu
Kyoto Institute Of Nutrition And Pathology Inc.
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ISHIZUKA Satoshi
Laboratory of Nutritional Biochemistry, Department of Bioscience and Chemistry, Faculty of Agriculture, Hokkaido University
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ISHIZUKA Satoshi
Laboratory of Food Biochemistry, Department of Bioscience and Chemistry, Faculty of Agriculture, Hokkaido University
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Tsukahara Takamitsu
Kyoto Institute of Nutrition and Pathology, Inc.
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