Development of a Method for the Identification of S-IgA-Coated Bacterial Composition in Mouse and Human Feces
スポンサーリンク
概要
- 論文の詳細を見る
Some commensal intestinal bacteria in humans and mice are coated with secretory immunoglobulin A (S-IgA). It has been suggested that the S-IgA coating of commensal bacteria does not occur at random and thus identification of S-IgA-coated bacterial genera/species should help in elucidating the interaction between S-IgA and commensal intestinal bacteria, but no method of identifying the genera/species of S-IgA-coated bacteria has been established. To identify S-IgA-coated bacterial composition, we developed a method combining immunohistochemical detection of S-IgA and subsequent 16S rRNA targeted fluorescence in situ hybridization (FISH) analysis. Human and mouse fecal S-IgA coated bacterial composition was evaluated by this newly developed method with 10 frequently-used FISH probes. Fecal S-IgA-coated bacterial composition was successfully analyzed by this method, and this suggests that Enterobacteriaceae is preferably coated with S-IgA, whereas Bacteroides/Prevotella and Lactobacillus/Enterococcus groups appear to be poorly coated with S-IgA.
- 2010-05-23
著者
-
HARA HIROSHI
Laboratory of Nutritional Biochemistry, Graduate School of Agriculture, Hokkaido University
-
Yajima Takaji
Division Of Research And Development Meiji Dairies Corporation
-
Tsuruta Takeshi
Laboratory of Nutritional Biochemistry, Department of Applied Bioscience, Graduate School of Agricul
-
Inoue Ryo
Laboratory Of Animal Science Kyoto Prefectural University
-
Iwanaga Toshihiko
Laboratories Of Anatomy Graduate School Of Veterinary Medicine Hokkaido University
-
Yajima Takaji
Meiji Dairies Research Chair Creative Research Initiative Sousei Hokkaido University
-
Tsuruta Takeshi
Laboratory Of Nutritional Biochemistry Department Of Applied Bioscience Graduate School Of Agricultu
-
Tsuruta Takeshi
Laboratory Of Animal Science Kyoto Prefectural University
-
Hara Hiroshi
Laboratory Of Nutritional Biochemistry Division Of Applied Bioscience Graduate School Of Agriculture
-
Hara Hiroshi
Lab. Of Nutritional Biochemistry Div. Of Applied Bioscience Graduate School Of Agriculture Hokkaido
関連論文
- Effects of difructose anhydride III (DFA III) administration on rat intestinal microbiota.
- Localization of Five Steroidogenic Enzyme mRNAs in Japanese Black Bear (Ursus thibetanus japonicus) Testes During the Mating Season by In Situ Hybridization
- Effects of germinated barley foodstuff on dextran sulfate sodium-induced colitis in rats
- Lactic Acid Bacteria (LAB) Bind to Human B- or H-Antigens Expressed on Intestinal Mucosa
- Cellular Distribution of Napsin (Kidney-Derived Aspartic Protease-like Protein, KAP) mRNA in the Kidney, Lung and Lymphatic Organs of Adult and Developing Mice
- Development of Intestinal Microbiota in Mice and Its Possible Interaction with the Evolution of Luminal IgA in the Intestine
- Adrenaline-Induced Lipolysis in Isolated Rat Mesenteric Adipocytes Is Higher in the Large Intestine Than in the Small Intestine
- High Biliary Excretion Levels of Quercetin Metabolites after Administration of a Quercetin Glycoside in Conscious Bile Duct Cannulated Rats
- A soluble flavonoid-glycoside, alphaG-rutin, is absorbed as glycosides in the isolated gastric and intestinal mucosa.
- Strain Gauge Force Transducer and Its Application in a Pig Model to Evaluate the Effect of Probiotic on Colonic Motility
- Lactobacillus casei Strain Shirota-fermented Milk Stimulates Indigenous Lactobacilli in the Pig Intestine
- Development of a Method for the Identification of S-IgA-Coated Bacterial Composition in Mouse and Human Feces
- Long-term oral administration of cows' milk improves insulin sensitivity in rats fed a high-sucrose diet
- Voluntary Running Exercise Alters Microbiota Composition and Increases n-Butyrate Concentration in the Rat Cecum
- Immunohistochemical Localization of Napsin and Its Potential Role in Protein Catabolism in Renal Proximal Tubules
- Increased concentrations of protein gene product 9.5 in the synovial fluid from horses with osteoarthritis
- Immunohistochemical demonstration of chromogranin A in endocrine organs of the rat and horse by use of region-specific antibodies
- Effect of germinated barley foodstuff administration on mineral utilization in rodents
- Growth Potencies and Effects on Digestive Functions of an Amino Acid and a Casein Diet in Rats of Three Strains
- Expression of galanin receptor-1 (GALR1) in the rat trigeminal ganglia and molar teeth
- The expression of src-suppressed C kinase substrate (SSeCKS) and uptake of exogenous particles in endothelial and reticular cells
- Passive immunization with monoclonal antibodies : effects on Haemaphysalis longicornis tick infestation of BALB/c mice
- Cellular architecture of the synovium in the tendon sheath of horses : An immunohistochemical and scanning electron microscopic study
- Cellular Localization of the Diazepam Binding Inhibitor in Glial Cells with Special Reference to Its Coexistence with Brain-type Fatty Acid Binding Protein
- Cellular Localization of the Diazepam Binding Inhibitor (DBI) in the Gastrointestinal Tract of Mice and Its Coexistence with the Fatty Acid Binding Protein (FABP)
- Porosity of the Epithelial Basement Membrane as an Indicator of Macrophage-Enterocyte Interaction in the Intestinal Mucosa
- Effects of DFA IV in Rats : Calcium Absorption and Metabolism of DFA IV by Intestinal Microorganisms
- Improvement of Bone Strength and Dermal Thickness Due to Dietary Edible Bird’s Nest Extract in Ovariectomized Rats
- Immunohistochemical Demonstration of Nerve Terminals in the Whole Hard Palate of Rats by Use of an Antiserum against Protein Gene Product 9.5 (PGP 9.5)
- Immunohistochemical Identification of Type B Intercalated Cells in the Rat Kidney by a Monoclonal Antibody
- Immunoreactivity to a Monoclonal Antibody (OS-3) Is Shared by Osteoclasts and Bicarbonate-Secreting Cells
- Morphology and Functional Roles of Synoviocytes in the Joint
- Diverse Immune Responses to Orally Administered Heat-Killed Cell Preparation of Enterococcus faecalis Strain EC-12 in Murine Immune Tissues
- Oral Administration of a Heat-Killed Cell Preparation of Enterococcus faecalis Strain EC-12 Stimulates the Gene Expression of Na^+-K^+-2Cl^- Co-Transporter 1 in Rat Ileal Epithelial Cells
- Three-dimensional Ultrastructure of Synoviocytes in the Knee Joint of Rabbits and Morphological Changes in Osteoarthritis Model
- Development of a Method for the Identification of S-IgA-Coated Bacterial Composition in Mouse and Human Feces
- Voluntary Running Exercise Alters Microbiota Composition and Increases n-Butyrate Concentration in the Rat Cecum
- A MONOCLONAL ANTIBODY, 169.1,AGAINST CANINE LEUKOCYTE SURFACE ANTIGEN IDENTIFIES CYTOSKELETAL COMPONENTS IN EPITHELIAL CELLS AND PERIPHERAL NEURONS
- Distribution of neutral amino acid transporter ASCT1 in the non-neuronal tissued of mice
- Possible Link of a Compositional Change in Intestinal Microbiota with the Anti-Allergic Effect of Fructo-Oligosaccharides in NC/jic Mice
- A cell preparation of Enterococcus faecalis strain EC-12 stimulates the luminal immunoglobulin A secretion in juvenile calves
- Improved Isolation Methods for Mucosal Leukocytes from Small and Large Intestines in Rats
- High Biliary Excretion Levels of Quercetin Metabolites after Administration of a Quercetin Glycoside in Conscious Bile Duct Cannulated Rats
- Adrenaline-Induced Lipolysis in Isolated Rat Mesenteric Adipocytes Is Higher in the Large Intestine Than in the Small Intestine
- Improvement of Bone Strength and Dermal Thickness Due to Dietary Edible Bird's Nest Extract in Ovariectomized Rats
- Parasitic forms of a myxosporean in the kidney of the arctic lamprey, Lampetra japonica : An ultrastructural study
- Strain Gauge Force Transducer and Its Application in a Pig Model to Evaluate the Effect of Probiotic on Colonic Motility
- The Large Intestine Compensates for Insufficient Calcium Absorption in the Small Intestine in Rats
- Supplementation of Methionine to a Low Soybean Protein Diet Strikingly Increases Pancreatic Amylase Activity in Rats
- Non-neuronal release of ACh plays a key role in secretory response to luminal propionate in rat colon
- Nicotine- and Tar-Free Cigarette Smoke Induces Cell Damage Through Reactive Oxygen Species Newly Generated by PKC-Dependent Activation of NADPH Oxidase
- Evaluation of the heat-killed and dried cell preparation of Enterococcus faecalis against villous atrophy in early-weaned mice and pigs
- Apoptosis of Enterocytes Induced by Inoculation of a Strain of Attaching and Effacing Escherichia coli and Verotoxin.
- Quantitative Changes in Serum Concentration of Bovine Gut Chitinase in Theileria Infection
- Morphological Analysis of Olfactory Receptor Cells Using Whole-Mount Preparations of the Rat Nasal Mucosa.
- A Rapid Method of Screening Lactic Acid Bacterial Strains for Conjugated Linoleic Acid Production