Increased concentrations of protein gene product 9.5 in the synovial fluid from horses with osteoarthritis
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概要
- 論文の詳細を見る
Our previous study established protein gene product 9.5 (PGP 9.5), a ubiquitin C-terminal hydrolase, as a specific cytochemical marker of synovial lining cells (type B synoviocytes) in the horse joint. The present study aimed to detect PGP 9.5 in the synovial fluid and shows that PGP 9.5 is a valuable marker of osteoarthritis in the horse. Immunohistochemical staining confirmed rich and consistent localization of PGP 9.5 immunoreactivity in the cytoplasm of synovial lining cells in the normal horse joint. Western blot analysis of synovial fluid from normal joints could detect a significant band corresponding to that contained in the brain and synovial membrane extracts. When 60 synovial fluid samples from normal and abnormal joints were assayed with an enzyme-linked immunosorbent assay (ELISA) system, the concentration of PGP 9.5 tended to be elevated in osteochondrosis dissecance, inflammatory arthropathy and intra-articular fracture, among which a statistically significant elevation was recognizable between the intra-articular fracture and the control. Thus, this study demonstrated the possibility that PGP 9.5, derived from synovial lining cells, may be a new biochemical marker for arthritic disorders of the horse.
- 北海道大学の論文
- 2001-08-31
著者
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SATO Fumio
Laboratory of Racing Chemistry
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IWANAGA Toshihiko
Laboratory of Histology and Cytology, Hokkaido University Graduate School of Medicine
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OKUMURA Masahiro
Laboratory of Veterinary Surgery, Department of Veterinary Clinical Sciences, Graduate School of Vet
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SATO Fumio
Department of Astronomy and Earth Sciences, Tokyo Gakugei University
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Okumura Masahiro
Laboratories Of Veterinary Surgery Graduate School Of Veterinary Medicine Hokkaido University
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Iwanaga Toshihiko
Laboratory Of Anatomy Department Of Biomedical Sciences Graduate School Of Veterinary Medicine Hokka
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Iwanaga Toshihiko
Laboratory Of Histology And Cytology Department Of Functional Morphology Graduate School Of Medicine
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Sato F
Equine Research Institute
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KITAMURA Hiroko
Laboratory of Anatomy, Department of Biomedical Sciences, Graduate School of Veterinary Medicine, Ho
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KIMOTO Keita
Laboratory of Anatomy, Department of Biomedical Sciences, Graduate School of Veterinary Medicine, Ho
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KOHAMA Morimasa
Laboratory of Anatomy, Department of Biomedical Sciences, Graduate School of Veterinary Medicine, Ho
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HASHIMOTO Yoshiharu
Laboratory of Anatomy, Department of Biomedical Sciences, Graduate School of Veterinary Medicine, Ho
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TAGAMI Masaaki
Shadai Horse Clinic
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SATO Fumio
NHK Science and Technical Research Laboratories
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Sato F
Japan Racing Association Utsunomiya Jpn
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Iwanaga T
Hokkaido Univ. Sapporo
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Sato F
Nhk Science And Technical Research Laboratories
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Iwanaga T
Laboratory Of Histology And Cytology Hokkaido University Graduate School Of Medicine
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Iwanaga Toshihiko
Laboratories Of Anatomy Graduate School Of Veterinary Medicine Hokkaido University
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Kimoto Keita
Laboratory Of Anatomy Department Of Biomedical Sciences Graduate School Of Veterinary Medicine Hokka
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Hashimoto Yoshiharu
Laboratory Of Anatomy Department Of Biomedical Science Graduate School Of Veterinary Medicine Hokkai
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Okumura Masahiro
Laboratory Of Veterinary Surgery Department Of Veterinary Clinical Sciences Graduate School Of Veter
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Kohama Morimasa
Laboratory Of Anatomy Department Of Biomedical Sciences Graduate School Of Veterinary Medicine Hokka
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Kitamura H
Laboratory Of Anatomy Department Of Biomedical Sciences Graduate School Of Veterinary Medicine Hokka
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Kitamura Hiroko
Laboratory Of Anatomy Department Of Biomedical Sciences School Of Veterinary Medicine Hokkaido Unive
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SATO Fumio
Laboratory of Molecular and Cellular Biology, Equine Research Institute, Japan Racing Association
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