IMMUNOCHEMICAL APPROACH TO INSULIN RECEPTOR WITH USE OF SYNTHETIC PEPTIDES
スポンサーリンク
概要
- 論文の詳細を見る
Based on the predicted amino acid sequence of human placental insulin receptor protein (HIRP), we prepared five synthetic peptides corresponding to purposely selected regions of HIRP; (9-25), (30-61), (48-71), (736-760) and (1139-1171). Antisera were generated against the respective synthetic peptides. Radioimmunoassays with anti-HIRP (30-61) and anti-HIRP (1139-1171) sera could recognize two forms, 270K and 850K daltons, of solubilized insulin receptor in gel filtration fractions, presumably HIRP aβ-monomeric and its tetrameric forms. Immunostaining with the five antisera against the synthetic HIRP fragments revealed positive cells in human placental and hepatic tissues; particularly the antisera stained strongly the membrane regions of Hofbauer cellsin the placenta. The results support the validity and usefulness of the region-specific anti-synthetic HIRP fragment sera in studies on the molecular basis of insulin receptor.
- 日本組織細胞化学会の論文
著者
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Iguchi Kazuaki
Laboratory of Bioorganic Chemistry, School of Pharmaceutical Sciences, University of Shizuoka
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Iguchi Kazuaki
Laboratory Of Bioorganic Chemistry School Of Pharmaceutical Sciences University Of Shizuoka
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Iguchi Kazuaki
Lab. Of Bioorganic Chemistry School Of Pharmaceutical Sciences Univ. Of Shizuoka
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Hoshino Minoru
Laboratory Of Bioorganic Chemistry School Of Pharmaceutical Sciences University Of Shizuoka
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YANAIHARA CHIZUKO
Shizuoka College of Pharmacy
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MOCHIZUKI TOHRU
Shizuoka College of Pharmacy
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INOUE TAKASHI
Shizuoka College of Pharmacy
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IGUCHI KAZUAKI
Shizuoka College of Pharmacy
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T0Y0SHIGE MICHIHIRO
National Institute for Physiological Sciences
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HOSHINO MINORU
National Institute for Physiological Sciences
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IWANAGA TOSHIHIKO
Niigata University School of Medicine
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FUJITA TSUNEO
Niigata University School of Medicine
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IZUMI SHINICHI
Tokai University School of Medicine
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NAKANE KAZUHO
Tokai University School of Medicine
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YANAIHARA NOBORU
Shizuoka College of Pharmacy
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Moriuchi Tetsuya
Hokkaido Urothelial Cancer Research Group
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Inoue S
Frontier Device Research Center Seiko Epson Corporation
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Moriuchi Tetsuya
Department Of Cell Biology Tokai University School Of Medicine
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Yanaihara N
Yanaihara Inst. Shizuoka Jpn
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Mochizuki T
Shizuoka Cancer Center Hospital And Res. Inst. Shizuoka Jpn
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Mochizuki Tohru
Shizuoka Cancer Center Hospital And Research Institute
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Yanaihara C
Hyogo Medical Univ. Nishinomiya‐shi Jpn
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