LOCALIZATION OF C-MYC IN HL-60 CELLS, NEOPLASTIC AND NORMAL TISSUES : AN IMMUNOHISTOCHEMICAL AND IN SITU HYBRIDIZATION STUDY
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概要
- 論文の詳細を見る
Our preliminary immunohistochemical examination revealed that c-myc protein was localized in the cytoplasm of both normal and neoplastic cells. This finding did not correlate with the reported nuclear localization of c-myc protein by other analyses. To clarify this discrepaney, we investigated whether the cytoplasmic localization of c-myc protein in our preliminary immunohistochemical studies reflects true physiological habitat of the protein or the results of an artifact which occurred during tissue processing. For the immunohistochemical localization of c-myc protein, rabbit antisera against synthetic peptides which correspond to a portion of c-myc were utilized. Fixation conditions were examined by varying the temperature, the salt concentration, as well as the duration of fixation. With conventional fixation conditions used for the routine immunohistochemical localization of proteins, such as fixation with a solution of 4% formaldehyde with low salt concentration at low temperature, the c-myc protein was found in the cytoplasm of HL-60 cells, some gastric carcinoma cells, intestinal crypt epithelial cells and spermatogonia in seminiferous tubules from human and regenerating hepatocytes from rat. Whereas, when these cells and tissues were fixed at 42℃ with a fixative containing 4% formaldehyde and a high concentration of NaCl, the c-myc protein was found in the nuclei of these cells. These results suggest that during fixation the c-myc protein translocates from nucleus to cytoplasm. To prove that these c-myc proteins were the product of the cells, in situ hybridization of c-myc mRNA was carried out using dinitrophenyl labeled c-myc cDNA. The gastric carcinoma cells which contained c-myc protein in their nuclei were morphologically similar to those contained the c-mye mRNA. The co-existance of c-myc protein and c-myc mRNA suggests that thc c-myc protein found in the nuclei was the product of the cell.
- 日本組織細胞化学会の論文
著者
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Koji Takehiko
Department Of Histology And Cell Biology Nagasaki University Graduate School Of Biomedical Sciences
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YANAIHARA Chizuko
Department of Pharmacy, Hyogo Medical University
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Iguchi Kazuaki
Laboratory of Bioorganic Chemistry, School of Pharmaceutical Sciences, University of Shizuoka
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Iguchi Kazuaki
Laboratory Of Bioorganic Chemistry School Of Pharmaceutical Sciences University Of Shizuoka
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Iguchi Kazuaki
Lab. Of Bioorganic Chemistry School Of Pharmaceutical Sciences Univ. Of Shizuoka
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TERASHIMA Kazuo
Department of Advanced Materials Science, Graduate School of Frontier Science, The University of Tok
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MOCHIZUKI Tohru
Department of Pharmaceutical Sciences of Shizuoka
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Nakane Paul
長崎大学 解剖3
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Abe Kaoru
Department Of Pathology Nagasaki University School Of Medicine
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Moriuchi Tetsuya
Hokkaido Urothelial Cancer Research Group
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Inoue S
Frontier Device Research Center Seiko Epson Corporation
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Izumi Shin-ichi
Department of Histology and Cell Biology, Nagasaki University School of Medicine
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NAKANE Paul
Department of Cell Biology, School of Medicine, Tokai University
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MORIUCHI Tetsuya
Department of Cell Biology, Tokai University School of Medicine
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Abe Kaoru
Department Of Endocrinology National Cancer Center Research Institute
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Terashima Kazuo
Department Of Advanced Materials Science Graduate School Of Frontier Science The University Of Tokyo
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Terashima Kazuo
Department Of Pathology Yamagata University
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YANAIHARA NOBORU
Department of Bioorganic Chemistry, Shizuoka College of Pharmacy
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Moriuchi Tetsuya
Department Of Cell Biology Tokai University School Of Medicine
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Koji Takehiko
Department Fo Histology And Cell Biology Nagasaki University School Of Medicine
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Abe K
Department Of Pathology Nagasaki University School Of Medicine
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NAKANE Paul
Environmental Biotechnology Institute, California Polytechnic State University
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TANNO MASASHI
Department of Cell Biology, Tokai University
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IGUCHI KAZUOKI
Department of Bio-organic Chemistry, Shizuoka College of Pharmacy
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Nakane Paul
Department Of Anatomy Nagasaki University School Of Medicine
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Tanno Masashi
Department Of Cell Biology Tokai University
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Abe Katsushige
Second Department Of Internal Medicine Nagasaki University Graduate School Of Biomedical Sciences
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Yanaihara N
Yanaihara Inst. Shizuoka Jpn
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Yanaihara Noboru
Department Of Bioorganic Chemistry Shizuoka College Of Pharmacy
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Izumi Shin-ichi
Department Of Anatomy Nagasaki University School Of Medicine
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Mochizuki T
Shizuoka Cancer Center Hospital And Res. Inst. Shizuoka Jpn
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Nakane P
Essential Therapeutics Ca Usa
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Yanaihara C
Hyogo Medical Univ. Nishinomiya‐shi Jpn
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Yanaihara Chizuko
Department Of Bioorganic Chemistry Shizuoka College Of Pharmacy
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Nakane Paul
Department Of Anatomy (iii) Nagasaki University School Of Medicine
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Yanaihara Noboru
Department Of Anatomy Hiroshima University School Of Medicine:laboratory Of Bioorganic Chemistry Shi
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Abe Kaoru
Second Department Of Internal Medicine Nagasaki University Graduate School Of Biomedical Sciences
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YANAIHARA CHIZUKO
Department of Bio-organic Chemistry, Shizuoka College of Pharmacy
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