Semi-Quantitative Non-Radioactive In Situ Hybridization and Its Clinical Application.

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概要

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• In situ hybridization (ISH) is widely used to determine the expression of specific mRNA in tissues. However, the results of ISH are influenced by the amount of retained RNA in the tissue. Estimation of retained RNA in the tissue is very important and the results of ISH should be interpreted taking into consideration the amount of retained RNA in samples. In the present study, we determined the amount of retained RNA by performing ISH for 28S rRNA. We also analyzed the results of ISH semi−quantitatively by computer image analysis. The method was applied by calculating the ratio of specific mRNA to 28S rRNA in order to standardize the results of ISH among different tissue samples. Our method is useful for comparing the expression of specific mRNA in different tissue samples with variable levels of retained RNA. Since the conditions of fixation and storage of tissues differ among samples, especially in clinical samples, our semi−quantitative method allows a more precise evaluation of gene expression in different samples.

• 日本組織細胞化学会の論文

著者

• Kohno Shigeru

  the Second Department of Internal Medicine, Nagasaki University, School of Medicine

• Harada Takashi

  Division Of Nephrology Clinical Research Center National Kyushu Medical Center

• Koji Takehiko

  Department Of Histology And Cell Biology Nagasaki University Graduate School Of Biomedical Sciences

• SUZUKI Daisuke

  Department of Anatomy, Sapporo Medical University School of Medicine

• SAKAI Hideto

  Department of Internal Medicine, School of Medicine, Tokai University

• Furusu Akira

  長崎大学 医学部第二内科

• Furusu Akira

  Second Department Of Internal Medicine Nagasaki University Graduate School Of Biomedical Sciences

• Furusu Akira

  長崎大学 医学部内科学第2

• Miyazaki Masanobu

  Miyazaki clinic

• Sakai Hideto

  Department Of Intermal Medicine School Of Medicine Tokai University

• MIYAZAKI Masanobu

  the Second Department of Internal Medicine, Ngasaki University School of Medicine

• OZONO Yoshiyuki

  the Second Department of Internal Medicine, Ngasaki University School of Medicine

• Shin Masashi

  Department of Applied Biosciences, Sojo University School of Technology

• Tsukasaki Shoko

  The Second Department of Internal Medicine

• Abe Katsushige

  The Second Department of Internal Medicine

• Furusu Akira

  The Second Department of Internal Medicine

• Sakai Hideto

  Department Of Nephrology Tokai University

• Koji Takehiko

  Department Fo Histology And Cell Biology Nagasaki University School Of Medicine

• Harada Takashi

  Division Of Renal Care Unit Nagasaki University School Of Medicine

• Shin Masashi

  Department Of Histology And Cell Biology

• Abe Katsushige

  Second Department Of Internal Medicine Nagasaki University Graduate School Of Biomedical Sciences

• Miura Masahiko

  Department Of Internal Medicine School Of Medicine Tokai University

• Kohno Shigeru

  The Second Department Of Internal Medicine Nagasaki University

• Kohno Shigeru

  The Scientific Assembly For Infection And Tuberculosis Of The Japanese Respiratory Society

• Harada T

  Nec Corp. Kawasaki‐shi Jpn

• Abe Kaoru

  Second Department Of Internal Medicine Nagasaki University Graduate School Of Biomedical Sciences

• Suzuki Daisuke

  Department Of Anatomy Sapporo Medical University School Of Medicine

• Suzuki Daisuke

  Department Of Nephrology Tokai University

• Sakai Hideto

  Department Of Health Care Policy And Management Graduate School Of Comprehensive Sciences University Of Tsukuba

• OZONO Yoshiyuki

  The Second Department of Internal Medicine, Nagasaki University School of Medicine

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