Demonstration of Proliferating Cell Nuclear Antigen (PCNA/Cyclin) in Glioma Cells
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概要
- 論文の詳細を見る
Proliferating cell nuclear antigen (PCNA/cyclin), a non-histone, acidic nuclear protein commonly believed to be strictly confined to proliferating cells, was successfully demonstrated exclusively in the nuclei of human glioma cells both in vitro and in situ by an indirect immunoperoxidase method. Speckled nucleoplasmic staining for PCNA, with variable nucleolar staining, was observed in approximately 37% of cultured human glioma (KY) cells in exponential growth, whereas only 14% of subcutaneous KY tumor cells from inoculated nude mice stained unequivocally positive for nuclear PCNA. A direct comparison of this method and autoradiography would be valuable. However, immunocytochemical demonstration of PCNA appears to be a simple method of estimating the proliferative activity of glioma cells, since the number of PCNA-positive KY cells closely corresponds to the number of S phase cells that exhibit nuclear uptake of bromodeoxyuridine.
- 日本脳神経外科学会の論文
- 1987-01-15
著者
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TABUCHI Kazuo
Department of Neurosurgery, Saga Medical School
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Nakane Paul
長崎大学 解剖3
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Tabuchi Kazuo
小柳記念病院 脳神経外科
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Tabuchi K
Department Of Neurosurgery Saga Medical School
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Tabuchi Kazuo
Department Of Neurological Surgery Okayama University Medical School
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Tabuchi K
Department Of Neurosurgery Kagawa Medical School
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NAKANE Paul
Department of Cell Biology, School of Medicine, Tokai University
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NAKANE Paul
Environmental Biotechnology Institute, California Polytechnic State University
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Nakane Paul
Department Of Anatomy Nagasaki University School Of Medicine
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Nakane P
Essential Therapeutics Ca Usa
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HONDA Chiho
Department of Neurosurgery, Kagawa Medical School
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Honda Chiho
Department Of Neurosurgery Iwakuni National Hospital
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Honda Chiho
Department Of Neurosurgery Kagawa Medical School
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Nakane Paul
Department Of Anatomy (iii) Nagasaki University School Of Medicine
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