CYTOSKELETAL PROTEIN CHANGES IN HEPATOCYTES OF BILE DUCT-LIGATED RATS

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概要

• 論文の詳細を見る

• Intermediate filament-rich cytoskeletal protein was extracted from normal and 7 or 21-day bite duct-ligated rat hepatocytes, and analyzed by both one and two-dimensional gel electrophoresis. The most marked change in the bile duct-ligated rats was an increase in 44 kd polypeptide, determined by immunoblotting to be actin. Cytokeratin, the intermediate filament type found in hepatocytes, was also slightly increased. However, a marked decrease of the basic side spot of 55 kd paired cytokeratin components was noted on two-dimensional get electrophoresis. Fluorescent staining of liver tissue F-actin using NBD-phallacidin showed no remarkable difference between the two groups. Therefore, the increased actin content of hepatocyte after bile duct ligation may be mainly G-actin rather than F-actin. These changes in hepatocyte cytoskeleton seem to be a cellular adaptation to the microenvironment.

• 日本組織細胞化学会の論文

著者

• Hoshino Minoru

  Laboratory Of Bioorganic Chemistry School Of Pharmaceutical Sciences University Of Shizuoka

• Miyaji M

  Research Center For Pathogenic Fungi And Microbial Toxicoses Chiba University

• Miyaji Makoto

  First Department Of Internal Medicine Nagoya City University Medical School

• Katagiri Kenji

  First Department Of Internal Medicine Nagoya City University Medical School

• TAKEUCHI Toshihiko

  First Department of Internal Medicine,Nagoya City University Medical School

• Ohnishi Hayato

  First Department of Internal Medicine, Nagoya City University Medical School

• Hoshino Makoto

  First Department of Internal Medicine, Nagoya City University Medical School

• Hayakawa Tomihiro

  First Department of Internal Medicine, Nagoya City University Medical School

• Takeshima Akihiko

  First Department of Internal Medicine, Nagoya City University Medical School

• Nakai Tomio

  First Department of Internal Medicine, Nagoya City University Medical School

• Nakai Tomio

  First Department Of Internal Medicine Nagoya City University Medical School

• Ohnishi Hayato

  First Department Of Internal Medicine Nagoya City University Medical School

• Takeuchi T

  Faculty Of Care And Rehabilitation Seijoh University

• Takeuchi Toshihiko

  First Department Of Internal Medicine Nagoya City University Medical School

• Hayakawa Tomihiro

  First Department Of Internal Medicine Nagoya City University Medical School

• Takeuchi Terumi

  Department Of Anatomy School Of Medicine Fujita-gakuen Health University

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