The Effect of Methyl Linoleate Hydroperoxides and Radical Initiator 2, 2-Azobis (2-Amidinopropane) Dihydrochloride on Antibody Production by Mouse Spleen Cells

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Methyl linoleate hydroperoxides (MLHPO) and 2, 2-azo-bis(2-amidinopropane)dihydrochloride (AAPH), a widely used free rad-ical initiator, were examined for effects on antibody production by spleen cells using plaque-forming cell response against sheep red blood cells (SRBC). The in vitro primary antibody response was enhanced in the presence of MLHPO at a concentration range of 2-20μM or AAPH at 0.1 #M, but was suppressed with higher concentrations of these compounds. In the in vitro secondary antibody response, both MLHPO and AAPH failed to increase plaque-forming cell response above that of the control culture. Following the oral administration of MLHPO (2.29 g/kg) four times to mice, in vivo primary plaque-forming cell response was signifi-cantly suppressed. After, a single intraperitoneal injection of AAPH (60 mg/kg) to mice, in vivo primary plaque-forming cell response was also suppressed. These findings suggest that primary antibody response can be affected by lipid hydroperoxides and oxygen radicals in vivo.
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