Secretion of Bax-Like Protein into Systemic Circulation from the Posterior Pituitary in the Rat Hypothalamo-Posterior Pituitary System
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概要
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To investigate the possibility of the neurosecretion of Bax-like protein in the adult rat, we examined its distribution in the hypothalamo-posterior pituitary system using light and electron microscopic immunohistochemistry. We also measured the serum Bax-like protein using an enzyme immunoassay. Neurons in the paraventricular nucleus (PVN) and supraoptic nucleus of the hypothalamus exhibited intense Bax-like immunoreactivity (Bax-IR). Bax-like immunoreactive axons projected to the posterior pituitary through the internal layer of the median eminence. By immunoelectron microscopy, Bax-IR was observed in the granulated vesicles and in the membrane of the rough-surfaced endoplasmic reticulum in the neuronal perikarya in the medial magnocellular division of PVN. It was also detected in the secretory vesicles in the nerve terminals of the posterior lobe of the pituitary gland. Double-labeling histochemistry revealed that almost all Bax-like immunoreactive substances were present in the oxytocin neurons, and many Bax-like immunoreactive neurons were nitro-oxidergic neurons. The enzyme immunoassay revealed that the average Bax-like protein concentration in the serum was 39.0±9.6 pmol/ml in the adult male rat. Colchicine treatment significantly increased the densities of Bax-IR in the PVN and decreased the serum Bax-like protein levels. From these results, Bax-like protein is considered to be produced in the hypothalamic oxytocin neurons, transported through axoplasmic flow and secreted into systemic circulation at the posterior pituitary as a "neurohormone".
- 日本組織細胞化学会の論文
著者
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Tanaka Yuetsu
Ryukyu Univ.
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TANAKA Masaki
Department of Health Care Chemistry, College of Environmental Health, Azabu University
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HAYASHI Seiji
Department of Earth and Planetary Sciences, Graduate School of Science, Nagoya University
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YANAIHARA Noboru
Yanaihara Institute Inc.
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Tanaka M
Department Of Anatomy Kyoto Prefectural University Of Medicine
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Tanaka Masaki
京都府立医科大学 第2解剖
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Tamada Yoshitaka
Department of Anatomy, Osaka Dental University
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Tamada Yoshitaka
大阪歯科大学 解剖学
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Tamada Y
Department Of Anatomy Osaka Dental University
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YAMADA Hisao
Department of Anatomy and Cell Science, Kansai Medical University
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Yamada Hisao
Department Of Anatomy And Cell Science Kansai Medical University
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Ibata Yasuhiko
Office Of The President Kyoto Prefectural University Of Medicine
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Ibata Y
Kyoto Prefectural Univ. Medicine Kyoto Jpn
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Ibata Yasuhiko
Second Department Of Anatomy Kyoto Prefectural University Of Medicine
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Ibata Yasuhiko
Department Of Anatomy Kyoto Prefectural University Of Medicine
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Ibata Yasuhiko
Department Of Anatomy And Neurobiology Kyoto Prefectural University Of Medicine
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Matsuda Tomoyuki
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Toho University
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Nagasawa Shingo
Yanaihara Institute Inc.
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KUROKAWA Kiyoshi
Department of Anatomy, Shiga University of Medical Science
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KUDO Motoi
Department of Anatomy, Shiga University of Medical Science
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Utsunomiya Kazuhiro
Department of Anatomy and Cell Science, Kansai Medical University
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Ibata Yasuhiko
2nd Department Of Anatomy Kyoto Prefectural University Of Medicine
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Iijima Norio
Department of Anatomy and Neurobiology, Kyoto Prefectural University of Medicine
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Kataoka Kiyoshi
Department of Physiology, Ehime University School of Medicine
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Tamura Yasuhisa
Department Of Anatomy And Cell Science Kansai Medical University
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Hisa Y
Otolaryngology-head And Neck Surgery Graduate School Of Medical Science Kyoto Prefectural University
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Kataoka Kiyoshi
Department Of Physiology Ehime University School Of Medicine Shigenobu
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Tamada Yoshitaka
Department Of Anatomy And Cell Science Kansai Medical University
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Kudo Motoi
Department Of Anatomy Shiga University Of Medical Science
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Iijima N
Department Of Anatomy Kyoto Prefectural University Of Medicine
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Tanaka Masaki
Department Of Health Care Chemistry College Of Environmental Health Azabu University
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Hayashi Seiji
Department Of Anatomy And Neurobiology Kyoto Prefectural University Of Medicine
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Ibata Yasuhiko
Dept.of Anatomy And Neurobiology Kyoto Prefectural Univ.of Medicine
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Hayashi S
Takushoku Univ. Tokyo Jpn
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Kurokawa K
Department Of Anatomy Shiga University Of Medical Science
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Kakihara K
Department Of Anatomy Shiga University Of Medical Science
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Yamada Hisao
Department Of Anatomy And Cell Science Kmu 21c Coe Project Kansai Medical University
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Yanaihara Noboru
Yanaihara Institute
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Kurokawa Kiyoshi
Department Of Anatomy School Of Medicine
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Matsuda Tomoyuki
Department Of Pharmacology Toho University Faculty Of Pharmaceutical Sciences
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Matsuda Tomoyuki
Department Of Anesthesiology And Anatomy And Neurobiology Kyoto Prefectural University Of Medicine
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Tanaka Masaki
Department Of Anatomy & Neurobiology Kyoto Prefectural University Of Medicine
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Yamada Hisao
Department Of Anatomy And Cell Biology Shiga University Of Medical Science
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Utsunomiya Kazuhiro
Department Of Anatomy And Cell Science Kansai Medical University
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IIJIMA Norio
Department of Anatomy and Neurobiology, Graduate School of Medicine, Nippon Medical School
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