Neural Activity-Dependent Cellular Proliferation in the Rat Cerebral Cortex
スポンサーリンク
概要
- 論文の詳細を見る
In recent years, studies on cell regeneration in the adult mammalian central nervous system have brought about the possibility of new strategies for treating neurological disorders. Many researchers have examined molecular/cellularbased approaches in vitro, while little has been determined about the modulatory processes affecting cellular proliferation and differentiation in vivo. We report here a new strategy to investigate cellular proliferation, differentiation, and regeneration using an experimental animal model for functional activation of the cerebral cortex in rats. The unilateral cerebral cortex was extensively activated by induction of cortical spreading depression (SD), characterized as propagation of neuronal/glial membrane depolarization. Cellular proliferation in the animals was assessed using histochemical studies with BrdU, and analog of thymidine. SD resulted in a dramatic increase in BrdU-labeled cells in the cortical hemisphere that had undergone the SD, depending on the number of elicited SD events. Immunohistochemical studies revealed that 53% of the BrdU-labeled cells in the SD-generated cortex were immunopositive for NG2, a maker of oligodendrocyte progenitor cells, while 25% were immunopositive for OX-42, a maker of microglia, at 3 days after SD. These studies indicate that neural activation induces cellular proliferation in the cerebral cortex, and that the cells subsequently differentiate into glial progenitors or microglia within 3 days.
- 日本組織細胞化学会の論文
著者
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Tanaka Yuetsu
Ryukyu Univ.
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Tamada Yoshitaka
大阪歯科大学 解剖学
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Tamada Y
Department Of Anatomy Osaka Dental University
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Tamada Yoshitaka
大阪歯科大学 解剖学-組織学
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YAMADA Hisao
Department of Anatomy and Cell Science, Kansai Medical University
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Yamada Hisao
Department Of Anatomy And Cell Science Kansai Medical University
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KATAOKA Yosky
Department of Anatomy and Cell Science, Kansai Medical University
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TAMURA Yasuhisa
Department of Anatomy and Cell Science, KMU 21C COE Project, Kansai Medical University
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CUI Yilong
Department of Anatomy and Cell Science, KMU 21C COE Project, Kansai Medical University
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Tamura Yasuhisa
Department Of Anatomy And Cell Science Kansai Medical University
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Hisa Y
Otolaryngology-head And Neck Surgery Graduate School Of Medical Science Kyoto Prefectural University
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Tamada Yoshitaka
Dept.of Anatomy And Neurobiology Kyoto Prefectural Univ.of Medicine
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Yamada H
Department Of Anatomy And Cell Science Kansai Medical University
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Cui Yilong
Department Of Anatomy And Cell Science Kmu 21c Coe Project Kansai Medical University
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Kataoka Y
Department Of Anatomy And Cell Science Kansai Medical University
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Hamada Yoshinori
Department Of Anatomy And Cell Science Kansai Medical University
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Yamada Hisao
Department Of Anatomy And Cell Science Kmu 21c Coe Project Kansai Medical University
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Yamada Hisao
Department Of Anatomy And Cell Biology Shiga University Of Medical Science
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