Generation of Transgenic Rats with YACs and BACs: Preparation Procedures and Integrity of Microinjected DNA
スポンサーリンク
概要
- 論文の詳細を見る
The aim of the present study was to investigate differences in the methods for preparing a large DNA fragment to be used for making transgenic rats from the standpoint of transgenic production efficiency and integrity of the introduced gene. In yeast artificial chromosome (YAC) transgenesis, three methods for preparing DNA for microinjection were compared: amplification of YAC in yeast (AMP), amplification of YAC in yeast and removal of the amplification element (AMP/RE), and no amplification of the YAC in yeast (AMP-). Production efficiency per microinjected ovum with DNA by the AMP method was four times higher than that by the AMP/RE and AMP-. Based on these results, we favor the AMP method in spite of the thymidine kinase gene-induced male sterility. In bacterial artificial chromosome (BAC) transgenesis, linear DNA fragments for microinjection prepared by three kinds of purification procedures were compared: Not I digestion and CsCl gradient ultra-centrifugation (Prep. 1), CsCl gradient ultra-centrifugation, Not I digestion, gel electrophoresis, and β-agarase digestion (Prep. 2), and CsCl gradient ultra-centrifugation, Not I digestion, pulse field gel electrophoresis, and b-agarase digestion (Prep. 3). Although the efficiency of producing transgenic rats was similar with all these three DNA preparations, integration of the intact DNA fragment only occurred with the Prep. 3 procedure. We therefore favor the Prep. 3 method for preparing BAC DNA fragments. These results indicate that the method used to prepare a large DNA fragment such as YAC and BAC DNAs is important in order to produce transgenic rats with an intact transgene.
- 社団法人 日本実験動物学会の論文
著者
-
Fujiwara Yoshihiro
Department Of Medicine Osaka University Medical School
-
HIRABAYASHI Masumi
YS New Technology Institute, Inc.
-
Ueda Masatsugu
YS New Technology Institute Inc., Shimotsuga-gun, Tochigi 329-0512, Japan
-
ITO Kazumi
YS New Technology Institute, Inc.
-
Ueda M
The Ys Institute Inc.
-
Ueda Masatsugu
The Ys Inst. Inc.
-
Ueda Masatsugu
ワイエスニューテクノロジー研究所
-
HIRABAYASHI Masumi
National Institute for Physiological Sciences
-
Hirabayashi M
National Inst. For Physiological Sciences
-
Hirabayashi Masumi
自然科学研究機構生理学研究所
-
Hirabayashi Masumi
信州大学 繊維
-
Ito K
Ys New Technology Inst. Inc.
-
Kimura K
Research Institute Of Life Science Snow Brand Milk Products Co. Ltd.
-
Fujiwara Yoshihiro
Department Of Biological Resources And Environmental Science School Of Agricultural Science Nagoya U
-
TAKAHASHI Ri-ichi
YS New Technology Institute. Inc.
-
KODAIRA Kunihiko
Research Institute of Life Science Snow Brand Milk Products Co., Ltd.
-
KODAIRA Koko
Research Institute of Life Science Snow Brand Milk Products Co., Ltd.
-
Hirao Masao
Ina Research Laboratory Kitayama Labes Co. Ltd.
-
Takahashi R
The Ys Institute Inc.
-
Takahashi Ri-ichi
Ys New Technology Institute Inc.
-
Ueda Masatsugu
Ys New Technology Institute
-
TAKAHASHI Ri-ichi
The YS Institute Inc.
-
Fujiwara Yoshihiro
Department Of Anesthesiology Aichi Medical University
-
Kodaira Kunihiko
Research Institute Of Life Science Snow Brand Milk Products Co. Ltd.
-
Ito Kazumi
Ys New Technology Institute
-
Hirabayashi Masumi
Ys New Technology Institute Inc.
-
Fujiwara Yoshihiro
Ys New Technology Institute. Inc.
-
Ueda Masatsugu
The Ys Institute Inc.
-
Ueda Masatsugu
Ys New Tchnology Inst. Inc.
-
Ito Kazumi
YS New Technology Inst., Inc.
関連論文
- ラット卵子の自発的第一卵割に及ぼすCa2+/Mg2+不含培養液の影響
- 体外受精由来ウシ胚盤胞のガラス化保存においてバイオプシー後の培養時間が融解後の生存性に与える影響
- ラット体細胞核移植のためのストロンチウムによる卵子の活性化誘起
- Expression of Porcine FSHβ Subunit Promoter-driven Herpes Simplex Virus Thymidine Kinase Gene in Transgenic Rats
- ドナーとレシピエントのラット系統は1細胞期卵から体外発育した桑実胚〜胚盤胞の産仔発生に影響する
- Effects of 1-O-hexyl-2,3,5-trimethylhydroquinone on carbon tetrachloride-induced hepatic cirrhosis in rats
- ROLE OF MESANGIAL PROLIFERATION IN ANGIOTENSIN II AND ANTIDIURETIC HORMONE INDUCED CHANGES IN GLOMERULAR FILTRATION RATE
- THE FUNCTIONAL CHARACTERISTICS OF CULTURED RAT MESANGIAL CELL
- 円形精子細胞注入(ROSI)を施したラット卵子の個体発生に影響する要因
- 4Cで冷蔵保存された精巣上体から採取・凍結・融解した精子を用いたブタの繁殖の試み
- PJ-199 GDNF Acts as a More Potent Chemoattractant than NGF for Sympathetic Innervation in Cultured Ventricular Myocytes(Arrhythmia, basic(05)(A),Poster Session(Japanese),The 72nd Annual Scientific Meeting of the Japanese Circulation Society)
- FRS-020 Reconstitution of cardiac pacemaker sheets by the patterning of cell alignment using magnetic nanoparticles(New Insights into Basic Mechanism of Arrhythmia(A),Featured Research Session,The 72nd Annual Scientific Meeting of the Japanese Circulation
- Demethylation Dynamics of the Paternal Genome in Pronuclear-Stage Bovine Zygotes Produced by In Vitro Fertilization and Ooplasmic Injection of Freeze-Thawed or Freeze-Dried Spermatozoa
- Procedure for Bovine ICSI, not Sperm Freeze-drying, Impairs the Function of the Microtubule-organizing Center
- ラット細胞に顕微注入した卵丘細胞核の早期染色体凝集に影響する要因
- PROGNOSIS OF CHRONIC GLOMERULONEPHRITIS IN ADULT PATIENTS ESTIMATED ON THE BASIS OF THE MARKOV PROCESS
- CLINICAL STUDY ON IDIOPATHIC EDEMA, ESPECIALLY SYMPTOMS AND SIGNS : PROCEEDINGS OF THE 38TH ANNUAL MEETING OF THE JAPANESE CIRCULATION SOCIETY
- STUDIES ON A METAL-CHELATING ACTION OF FUROSEMIDE, ETHACRYNIC ACID AND BUMETANIDE : (Part II)
- Generation of Transgenic Rats with YACs and BACs: Preparation Procedures and Integrity of Microinjected DNA
- Transgenic Animals with Various YAC Constructs: Preparation of 400 kb YAC DNA for Microinjection
- A Comparative Study of the 5 Flanking Sequences of the αS1- and β-Casein Genes: Human and Other Mammalian Species
- Attempt at Intracytoplasmic Sperm Injection of In Vitro Matured Oocytes in Common Minke Whales (Balaenoptera acutorostrata) Captured During the Kushiro Coast Survey
- Maintenance of Transgenes in Rats : The Contributions of Embryo Cryopreservation and Intracytoplasmic Sperm/Spermatid Injection
- 前核注入法あるいは顕微授精法によるトランスジェニックラットの作製効率はリコンビナーゼを介在させても改善されない
- Production of Transgenic Rats Using Young Sprague-Dawley Females Treated with PMSG and hCG
- A comparative Study on the Integration of Exogenous DNA into Mouse, Rat, Rabbit, and Pig Genomes
- THE EFFECT OF SQ 14225 ON THE RESPONSE OF PLASMA ALDOSTERONE TO UPRIGHT POSITION IN PATIENT WITH MESANGIAL PROLIFERATIVE GLOMERULONEPHRITIS : Kidney : 46th Annual Scientific Meeting, Japanese Circulation Society
- Serum concentration of lidocaine after transversus abdominis plane block
- Anesthetic management of electroconvulsive therapy in a patient with a known history of neuroleptic malignant syndrome
- Refined Porcine Follicle Stimulating Hormone Promotes the Responsiveness of Rabbits to Multiple-Ovulation Treatment
- Development of a Conditionally Immortalized Testicular Sertoli Cell Line RTS3-3 from Adult Transgenic Rats Harboring Temperature-Sensitive Simian Virus 40 Large T-antigen Gene
- Development and Characterization of Conditionally Immortalized Gastric Epithelial Cell Lines from Transgenic Rats Harboring Temperature-Sensitive Simian Virus 40 Large T-antigen Gene
- Acidic Amino Acid Transport Characteristics of a Newly Developed Conditionally Immortalized Rat Type 2 Astrocyte Cell Line (TR-AST)
- Establishment of SV40-tsA58 Transgenic Rats as a Source of Conditionally Immortalized Cell Lines
- Cassette DNA Fragment for Selection and Sexing of Preimplantation Bovine Transgenic Embryos
- Viability of Transgenic Rat Embryos after Freezing and Thawing
- Transporter mRNA Expression in a Conditionally Immortalized Rat Small Intestine Epithelial Cell Line (TR-SIE)
- Establishment of Conditionally Immortalized Rat Retinal Pericyte Cell Lines (TR-rPCT) and Their Application in a Co-culture System Using Retinal Capillary Endothelial Cell Line (TR-iBRB2)
- トランスジェニックラットの作製効率におけるICSI経由法と前核注入法の直接比較
- Effects of Semen Extenders and Storage Temperatures on Characteristics of Frozen-Thawed Bryde's (Balaenoptera edeni) Whale Spermatozoa
- Effects of Semen Extenders and Storage Temperatures on Characteristics of Frozen-Thawed Brydes (Balaenoptera edeni) Whale Spermatozoa
- カタラーゼを添加したバッファー中でDNAを顕微注入すると胚の発育能力の低下を軽減できる
- Functional Expression of Human Growth Hormone (hGH) Transgene in the Rat Somatotroph
- Technical Development for Production of Gene-Modified Laboratory Rats
- Refined Porcine Follicle Stimulating Hormone Promotes the Responsiveness of Rabbits to Multiple-Ovulation Treatment
- Correlation between Erythropoietin and Lactate in Humans during Altitude Exposure
- CLOCK GENES EXPRESSION IN HEAD AND PERIPHERAL TISSUES OF BOMBYX MORI(Physiology,Abstracts of papers presented at the 75^ Annual Meeting of the Zoological Society of Japan)
- Dilute Kagome Lattice Magnetism with S=1/2 on Rb_2(Pd_M_x)_3S_4(M=Co, Mn)(Frustrated Systems, Field-Induced Phase Transitions and Dynamics in Quantum Spin Systems)
- Effect of flumazenil on recovery from sevoflurane anesthesia in children premedicated with oral midazolam before undergoing herniorrhaphy with or without caudal analgesia
- Enhanced colitis-associated colon carcinogenesis in a novel Apc mutant rat
- Donor and Recipient Rat Strains Affect Full-Term Development of One-Cell Zygotes Cultured to Morulae/Blastocysts
- Expression of Porcine FSHβ Subunit Promoter-driven Herpes Simplex Virus Thymidine Kinase Gene in Transgenic Rats
- Catalase in Manipulation Buffer Enhances the Developmental Competence of DNA-injected Embryos
- 体外受精由来ウシ胚盤胞のガラス化保存においてバイオプシー後の培養時間が融解後の生存性に与える影響
- 4Cで冷蔵保存された精巣上体から採取・凍結・融解した精子を用いたブタの繁殖の試み
- Transgenic Animals with Various YAC Constructs : Preparation of 400 kb YAC DNA for Microinjection
- Enhanced expression of naofen in kidney of streptozotocin-induced diabetic rats : possible correlation to apoptosis of tubular epithelial cells
- A Comparative Study of the 5' Flanking Sequences of the αS1- and β-Casein Genes : Human and Other Mammalian Species
- Erythropoietin gene expression by hydrogen peroxide
- O-5 Production of Transgenic Rats Showing Immunological tolerance to a Human Protein by the Introduction of a Fusion Gene Expressing under a Control of Milk Protein Gene Promoter.(Proceedings of the 27th Annual Meeting)
- Procedure for Bovine ICSI, not Sperm Freeze-drying, Impairs the Function of the Microtubule-organizing Center
- Human Hypoxic Signal Transduction through a Signature Motif in Hepatocyte Nuclear Factor 4
- Challenging Endeavour for Preservation of Freeze-Dried Mammalian Spermatozoa
- Challenging Endeavour for Preservation of Freeze-Dried Mammalian Spermatozoa
- Evaluation of left ventricular mechanical work and energetics of normal hearts in SERCA2a transgenic rats
- Establishment of tsA58 Transgenic Rats as a Source of Conditionally Immortalized Cell Lines with Differentiated Functions
- Role of naofen in apoptosis of hepatocytes induced by lipopolysaccharide through mitochondrial signaling in rats