Challenging Endeavour for Preservation of Freeze-Dried Mammalian Spermatozoa
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概要
- 論文の詳細を見る
Freeze-drying (lyophilization) has been proposed as an alternative method for sperm preservation to overcome the disadvantages of the current cryopreservation method such as the high maintenance cost of frozen stocks, the problems associated with transportation of frozen materials and the potential risk of total loss of the frozen stock. Since freeze-dried spermatozoa after rehydration lose their motility, which is an essential requirement to complete physiological fertilization, a relatively difficult microinsemination technique must be applied to rehydrated spermatozoa. Theoretically, it has been supposed that freeze-dried spermatozoa could maintain their functions and abilities to interact with the oocyte cytoplasm after prolonged storage at refrigerator temperature. However, sufficient yield of transferable blastocysts and production of live offspring derived from freeze-dried sperm samples are still subjects to be challenged and overcome in large domestic species.
- 日本繁殖生物学会の論文
著者
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HIRABAYASHI Masumi
National Institute for Physiological Sciences
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Abdalla Hany
Interdisciplinary Graduate School Of Science And Technology Shinshu University
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Hochi Shinichi
Fac. Of Textile Sci. And Technol. Shinshu Univ.
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Hochi Shinichi
Graduate School Of Sci. And Technol. Shinshu Univ.
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Hochi Shinichi
Graduate School Of Science And Technology Shinshu University
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Abdalla Hany
Graduate School Of Science And Technology Shinshu University
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Hara Hiromasa
Graduate School Of Science And Technology Shinshu University
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