トランスジェニックラットの作製効率におけるICSI経由法と前核注入法の直接比較
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概要
- 論文の詳細を見る
Production efficiency of transgenic rats was compared directly between the routine pronuclear microinjection of exogenous DNA solution (PNMI-Tg method) and the ooplasmic injection of sperm cells exposed to exogenous DNA solution (ICSI-Tg method) using six DNA constructs. The overall production efficiency per treated oocyte in the ICSI-Tg method (mean 1.1%, range 0.2 to 3.1%) was similar to that in the PNMI-Tg method (mean 1.1%, range 0 to 2.4%). An advantage of the ICSI-Tg method in the production of transgenic rats is noted in cases in which a low yield of pronuclear zygotes is an inevitable fate of the rat strain.
- 社団法人 日本実験動物学会の論文
著者
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平林 真澄
自然科学機構生理学研究所
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HOCHI Shinichi
Faculty of Textile Science and Technology, Shinshu University
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加藤 めぐみ
National Institute for Physiological Sciences
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HIRABAYASHI Masumi
National Institute for Physiological Sciences
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平林 真澄
National Institute for Physiological Sciences
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Hochi Shinichi
Faculty Of Textile Science And Technology Shinshu University
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KATO Megumi
National Institute for Physiological Sciences
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雨宮 和絵
National Institute for Physiological Sciences
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AMEMIYA Kazue
National Institute for Physiological Sciences
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