Production of Transgenic Rats Using Young Sprague-Dawley Females Treated with PMSG and hCG
スポンサーリンク
概要
- 論文の詳細を見る
The aim of this study was to examine the effects of gonadotrophin treatments on estrus synchronization and superovulation in young Sprague-Dawley (SD) rats that had not yet exhibited defined estrus cycles (5 to 7 weeks old), and to produce transgenic rats using these females as embryo donors and recipients. In Experiment 1, female rats were injected with PMSG and hCG (12.5, 25, 50 and 100 IU/kg each) and were mated with stud males. The reproductive performance of young rats were highest when PMSG and hCG at doses of 25 IU/kg each were injected (delivery rate 87.5%, nursing rate 92.9%). In Experiment 2, female rats were injected with PMSG and hCG (100, 150 and 300 IU/kg each) to induce superovulation. More eggs were recovered from the rats injected with PMSG and hCG at 150 and 300 IU/kg than from those treated with 100 IU/kg (33.4 and 41.3 vs. 13.3 eggs per female, respectively; p<0.05). In Experiment 3, pronuclear-stage zygotes from 150 IU/kg PMSG/hCG-treated rats were used for microinjection of the fusion gene of bovine αS1-casein gene promoter and human growth hormone gene (2.8 kb), and the microinjected zygotes were transferred into the oviduct ampullae of the 25 IU/kg PMSG/hCG-treated rats. Seventeen transgenic rats were obtained from the 334 DNA-injected zygotes (5.1%). These results indicate that recipients and embryo donors for the production of transgenic rats can be prepared by the appropriate PMSG and hCG treatments of young SD rats, regardless of their estrus stages.
- 社団法人 日本実験動物学会の論文
- 2001-10-01
著者
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HOCHI Shinichi
Faculty of Textile Science and Technology, Shinshu University
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Ueda Masatsugu
YS New Technology Institute Inc., Shimotsuga-gun, Tochigi 329-0512, Japan
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ITO Kazumi
YS New Technology Institute, Inc.
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Ueda M
The Ys Institute Inc.
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Ueda Masatsugu
The Ys Inst. Inc.
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Ueda Masatsugu
ワイエスニューテクノロジー研究所
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HIRABAYASHI Masumi
National Institute for Physiological Sciences
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Hirabayashi M
National Inst. For Physiological Sciences
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Hirabayashi Masumi
自然科学研究機構生理学研究所
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Hirabayashi Masumi
信州大学 繊維
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Ito K
Ys New Technology Inst. Inc.
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Hochi S
Laboratory Of Theriogenology Obihiro University Of Agriculture And Veterinary Medicine
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Hochi Shinichi
Faculty Of Textile Science And Technology Shinshu University
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HIRABAYSHI Masumi
YS New Technology Inst. Inc.
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SEKIMOTO Akiyo
YS New Technology Inst. Inc.
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Hirao Masao
Ina Research Laboratory Kitayama Labes Co. Ltd.
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Sekimoto A
Ys New Technology Inst. Inc.
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Ueda Masatsugu
Ys New Technology Institute
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Hochi S
Faculty Of Textile Science And Technology Shinshu University
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Ito Kazumi
Ys New Technology Institute
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Hirabayashi Masumi
Ys New Technology Institute Inc.
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Ueda Masatsugu
The Ys Institute Inc.
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Ueda Masatsugu
Ys New Tchnology Inst. Inc.
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Ito Kazumi
YS New Technology Inst., Inc.
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