Influence of Sera on <I>In Vitro</I> Hatching of Equine Blastocysts
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概要
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The influence of sera in culture medium on hatching of equine blastocysts was examined. Non-surgically recovered early blastocysts were cultured for 10 days in TCM199 supplemented with 10% (v/v) of: (1) fetal bovine serum (FBS, n=5), (2) fetal equine serum (FES, n=4), (3) mare serum collected at the day of ovulation (MS-O, n=6), or (4) mare serum collected at day 8 of pregnancy (MS-P, n=5). Mean embryonic diameters at the beginning of culture ranged from 165.3 to 177.3 μm. Embryos in MS-P started to hatch when their mean diameter reached 286.2 μm at day 8.6 of embryonic age. Embryos in MS-O, FES and FBS started to hatch at 322.5μm (day 10.0), 379.3μm (day 8.1) and 405.4μm (day 10.0), respectively. Judged from the maxi-mum diameter of the embryos during the culture period, fetal serum (FES: 1, 556.5μm; FBS: 942.2μm) supported the <I>in vitro</I> development of the embryos better than mare serum (MS-O; 677.5 μm, MS-P; 489.0μm). The hatching manner observed most frequently in FBS and MS-P supplemented media was a prolonged process characterized by either squeezing through a small opening in the zona pellucida or tearing open the zona pellucida. In contrast, a sudden escape from the zona pellucida was found in MS-O (3/5) and FES (2/4). The concentrations of E<SUB>2</SUB> and P<SUB>4</SUB> in FBS, FES, MS-O and MS-P were determined by EIA (E<SUB>2</SUB>: 0.074, 9.150, 0.010, and 0.013 ng/ml; P<SUB>4</SUB>: 0.800, 8.350, 2.390, and 6.340 ng/ml, respectively). In conclusion, the source of sera for supple-mentation of culture media influenced <I>in vitro</I> development of equine early blastocysts, especially the process of hatching.
- 日本繁殖生物学会の論文
著者
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Hochi S
Laboratory Of Theriogenology Obihiro University Of Agriculture And Veterinary Medicine
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OGURI Norihiko
Laboratory of Theriogenology, Obihiro University of Agriculture and Veterinary Medicine
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Hochi S
Faculty Of Textile Science And Technology Shinshu University
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HOCHI Shin-ichi
Laboratory of Horse Production, Obihiro University of Agriculture and Veterinary Medicine
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MIYAMOTO Akio
Animal Production Hygiene Department, Graduate School of Animal and Food Hygiene, Obihiro University
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Oguri Norihiko
Laboratory Of Horse Production Obihiro University Of Agriculture And Veterinary Medicine
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BRAUN Joachim
Laboratory of Horse Production
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FUKUI Yutaka
Animal Genetics and Reproduction, Obihiro University of Agriculture and Veterinary Medicine
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Hochi Shin-ichi
Laboratory Of Horse Production Obihiro University Of Agriculture And Veterinary Medicine
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MIYAMOTO Akio
Animal Genetics and Reproduction, Obihiro University of Agriculture and Veterinary Medicine
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OGURI Norihiko
Laboratory of Horse Production
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