体外受精由来ウシ胚盤胞のガラス化保存においてバイオプシー後の培養時間が融解後の生存性に与える影響
スポンサーリンク
概要
- 論文の詳細を見る
The aim of this study was to improve the cryosurvival of bovine blastocysts following biopsy. In vitro-derived bovine blastocysts were biopsied with a metal blade, then cultured for 0 to 20 h in m-SOF and then vitrified in the presence of ethylene glycol and dimethyl sulfoxide. The post-warm survival rates after 24 h of in vitro culturing of the biopsied embryos were significantly higher at 1.25, 2.5, 5 and 10 h than that of 0 and 20 h (69, 73, 78 and 64% vs 34 and 27%, p<0.05, respectively). The survival rate of embryos vitrified at 2.5 and 5 h after biopsy was not different from that of the non-vitrified control group (73 and 78% vs 96%, p=0.08 vs 0.07, respectively). These results indicate that 1.25 to 10 h culture between biopsy and vitrification improves the viability of bovine blastocysts produced in vitro.
- 日本繁殖生物学会の論文
- 1999-10-01
著者
-
伊藤 和美
YS New Technology Institute Inc.
-
HIRABAYASHI Masumi
YS New Technology Institute, Inc.
-
堰本 晃代
University Farm, Faculty of Agriculture, Utsunomiya University, Mohka, Tochigi 321-4415, Japan
-
平林 真澄
YS New Technology Institute Inc., Shimotsuga-gun, Tochigi 329-0512, Japan
-
Hochi Shinichi
Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, Ueda,
-
Kimura Ken
Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, Ueda,
-
Ueda Masatsugu
YS New Technology Institute Inc., Shimotsuga-gun, Tochigi 329-0512, Japan
-
Nagao Yoshikazu
University Farm, Faculty of Agriculture, Utsunomiya University, Mohka, Tochigi 321-4415, Japan
-
ITO Kazumi
YS New Technology Institute, Inc.
-
Ueda M
The Ys Institute Inc.
-
Ueda Masatsugu
The Ys Inst. Inc.
-
Ueda Masatsugu
ワイエスニューテクノロジー研究所
-
HIRABAYASHI Masumi
National Institute for Physiological Sciences
-
Hirabayashi M
National Inst. For Physiological Sciences
-
Hirabayashi Masumi
自然科学研究機構生理学研究所
-
Hirabayashi Masumi
信州大学 繊維
-
Ito K
Ys New Technology Inst. Inc.
-
Kimura K
Research Institute Of Life Science Snow Brand Milk Products Co. Ltd.
-
Hochi S
Laboratory Of Theriogenology Obihiro University Of Agriculture And Veterinary Medicine
-
Hochi Shinichi
Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
-
SEKIMOTO Akiyo
YS New Technology Inst. Inc.
-
Hirao Masao
Ina Research Laboratory Kitayama Labes Co. Ltd.
-
Ueda Masatsugu
Ys New Technology Institute
-
Sekimoto Akiyo
University Farm, Faculty of Agriculture, Utsunomiya University, Mohka, Tochigi 321-4415, Japan
-
Hochi S
Faculty Of Textile Science And Technology Shinshu University
-
Ito Kazumi
Ys New Technology Institute
-
Hirabayashi Masumi
Ys New Technology Institute Inc.
-
Nagao Yoshikazu
University Farm Faculty Of Agriculture Utsunomiya University
-
Ueda Masatsugu
The Ys Institute Inc.
-
Ueda Masatsugu
Ys New Tchnology Inst. Inc.
関連論文
- An Attempt to Predict the Response of Human Glutathione S-Transferase (GST) to Chemical Inducers Using Transgenic Rats Harboring Human GST Gene
- ラット卵子の自発的第一卵割に及ぼすCa2+/Mg2+不含培養液の影響
- 体外受精由来ウシ胚盤胞のガラス化保存においてバイオプシー後の培養時間が融解後の生存性に与える影響
- ラット体細胞核移植のためのストロンチウムによる卵子の活性化誘起
- Expression of Porcine FSHβ Subunit Promoter-driven Herpes Simplex Virus Thymidine Kinase Gene in Transgenic Rats
- ドナーとレシピエントのラット系統は1細胞期卵から体外発育した桑実胚〜胚盤胞の産仔発生に影響する
- 円形精子細胞注入(ROSI)を施したラット卵子の個体発生に影響する要因
- 4Cで冷蔵保存された精巣上体から採取・凍結・融解した精子を用いたブタの繁殖の試み
- PJ-199 GDNF Acts as a More Potent Chemoattractant than NGF for Sympathetic Innervation in Cultured Ventricular Myocytes(Arrhythmia, basic(05)(A),Poster Session(Japanese),The 72nd Annual Scientific Meeting of the Japanese Circulation Society)
- FRS-020 Reconstitution of cardiac pacemaker sheets by the patterning of cell alignment using magnetic nanoparticles(New Insights into Basic Mechanism of Arrhythmia(A),Featured Research Session,The 72nd Annual Scientific Meeting of the Japanese Circulation
- Demethylation Dynamics of the Paternal Genome in Pronuclear-Stage Bovine Zygotes Produced by In Vitro Fertilization and Ooplasmic Injection of Freeze-Thawed or Freeze-Dried Spermatozoa
- Procedure for Bovine ICSI, not Sperm Freeze-drying, Impairs the Function of the Microtubule-organizing Center
- ラット細胞に顕微注入した卵丘細胞核の早期染色体凝集に影響する要因
- Generation of Transgenic Rats with YACs and BACs: Preparation Procedures and Integrity of Microinjected DNA
- Transgenic Animals with Various YAC Constructs: Preparation of 400 kb YAC DNA for Microinjection
- A Comparative Study of the 5 Flanking Sequences of the αS1- and β-Casein Genes: Human and Other Mammalian Species
- Current Status of IVM/IVF/IVC Technology and Embryo Cryopreservation in the Equine Species
- Effects of Percoll Washing and Glycosaminoglycans on the Lysophosphatidylcholin Induced Acrosomal Reaction in Stallion
- Attempt at Intracytoplasmic Sperm Injection of In Vitro Matured Oocytes in Common Minke Whales (Balaenoptera acutorostrata) Captured During the Kushiro Coast Survey
- Maintenance of Transgenes in Rats : The Contributions of Embryo Cryopreservation and Intracytoplasmic Sperm/Spermatid Injection
- 前核注入法あるいは顕微授精法によるトランスジェニックラットの作製効率はリコンビナーゼを介在させても改善されない
- Production of Transgenic Rats Using Young Sprague-Dawley Females Treated with PMSG and hCG
- A comparative Study on the Integration of Exogenous DNA into Mouse, Rat, Rabbit, and Pig Genomes
- V-1 食道閉鎖症に合併した気管軟化症 : cine CTによる評価
- Follow-up survey of a large-scale multicenter, double-blind study of triple therapy with lansoprazole, amoxicillin, and clarithromycin for eradication of Helicobacter pylori in Japanese peptic ulcer patients
- Refined Porcine Follicle Stimulating Hormone Promotes the Responsiveness of Rabbits to Multiple-Ovulation Treatment
- Development of a Conditionally Immortalized Testicular Sertoli Cell Line RTS3-3 from Adult Transgenic Rats Harboring Temperature-Sensitive Simian Virus 40 Large T-antigen Gene
- Development and Characterization of Conditionally Immortalized Gastric Epithelial Cell Lines from Transgenic Rats Harboring Temperature-Sensitive Simian Virus 40 Large T-antigen Gene
- Acidic Amino Acid Transport Characteristics of a Newly Developed Conditionally Immortalized Rat Type 2 Astrocyte Cell Line (TR-AST)
- Establishment of SV40-tsA58 Transgenic Rats as a Source of Conditionally Immortalized Cell Lines
- Cassette DNA Fragment for Selection and Sexing of Preimplantation Bovine Transgenic Embryos
- Viability of Transgenic Rat Embryos after Freezing and Thawing
- Transporter mRNA Expression in a Conditionally Immortalized Rat Small Intestine Epithelial Cell Line (TR-SIE)
- Establishment of Conditionally Immortalized Rat Retinal Pericyte Cell Lines (TR-rPCT) and Their Application in a Co-culture System Using Retinal Capillary Endothelial Cell Line (TR-iBRB2)
- トランスジェニックラットの作製効率におけるICSI経由法と前核注入法の直接比較
- Effects of Semen Extenders and Storage Temperatures on Characteristics of Frozen-Thawed Bryde's (Balaenoptera edeni) Whale Spermatozoa
- Effects of Semen Extenders and Storage Temperatures on Characteristics of Frozen-Thawed Brydes (Balaenoptera edeni) Whale Spermatozoa
- カタラーゼを添加したバッファー中でDNAを顕微注入すると胚の発育能力の低下を軽減できる
- Functional Expression of Human Growth Hormone (hGH) Transgene in the Rat Somatotroph
- Technical Development for Production of Gene-Modified Laboratory Rats
- Refined Porcine Follicle Stimulating Hormone Promotes the Responsiveness of Rabbits to Multiple-Ovulation Treatment
- 形質転換ウシ受胎産物の作出:PCR法による形質転換胚の選別の可能性
- Correlation between Erythropoietin and Lactate in Humans during Altitude Exposure
- Laparoscopic cholecystectomy in gallstone patients with acute cholecystitis
- Enhanced colitis-associated colon carcinogenesis in a novel Apc mutant rat
- Donor and Recipient Rat Strains Affect Full-Term Development of One-Cell Zygotes Cultured to Morulae/Blastocysts
- An Attempt to Produce Acellular Embryonic Capsule in Equine Blastocysts Cultured In Vitro
- An Attempt to Produce Acellular Embryonic Capsule in Equine Blastocysts Cultured In Vitro
- Expression of Porcine FSHβ Subunit Promoter-driven Herpes Simplex Virus Thymidine Kinase Gene in Transgenic Rats
- Catalase in Manipulation Buffer Enhances the Developmental Competence of DNA-injected Embryos
- JS-II-6 EXTENSIVE AGANGLIONOSIS : Creation of A side-to-side Ileocolostomy for Prevention of Ileostomy Complications.
- 体外受精由来ウシ胚盤胞のガラス化保存においてバイオプシー後の培養時間が融解後の生存性に与える影響
- 4Cで冷蔵保存された精巣上体から採取・凍結・融解した精子を用いたブタの繁殖の試み
- Transgenic Animals with Various YAC Constructs : Preparation of 400 kb YAC DNA for Microinjection
- Influence of Relative Embryonic Volumes during Glycerol Equilibration on the Survival of Frozen-Thawed Equine Blastocysts
- Influence of Sera on In Vitro Hatching of Equine Blastocysts
- A Comparative Study of the 5' Flanking Sequences of the αS1- and β-Casein Genes : Human and Other Mammalian Species
- Erythropoietin gene expression by hydrogen peroxide
- CONCEPT OF SPECIFICATION FOR BIOPSY SITES
- O-5 Production of Transgenic Rats Showing Immunological tolerance to a Human Protein by the Introduction of a Fusion Gene Expressing under a Control of Milk Protein Gene Promoter.(Proceedings of the 27th Annual Meeting)
- Procedure for Bovine ICSI, not Sperm Freeze-drying, Impairs the Function of the Microtubule-organizing Center
- Human Hypoxic Signal Transduction through a Signature Motif in Hepatocyte Nuclear Factor 4
- Challenging Endeavour for Preservation of Freeze-Dried Mammalian Spermatozoa
- Challenging Endeavour for Preservation of Freeze-Dried Mammalian Spermatozoa
- Evaluation of left ventricular mechanical work and energetics of normal hearts in SERCA2a transgenic rats
- Questions Predicting Severe Disease in Patients with Abdominal Pain at a General Outpatient Department
- Infrared Observations of Polyethylene under the Condition of Electric Field Application