カタラーゼを添加したバッファー中でDNAを顕微注入すると胚の発育能力の低下を軽減できる
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概要
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To improve the efficiency of transgenesis, we investigated the effects of a radical scavenger during microinjection on the development to blastocysts or pups of mouse pronuclear embryos, microinjected with the enhanced green fluorescent protein (EGFP) transgene. When embryos were microinjected in medium containing 0-1,000 units/ml catalase, the developmental rate to blastocysts was significantly higher (P<0.01) in 100-units/ml catalase (81%) than those in 0 and 1,000 units/ml (56 and 65%). To investigate the ontogenetic ability of DNA-injected embryos, EGFP-injected embryos manipulated under 0 or 100 units/ml catalase were transferred separately to recipient mice. The proportion of fetuses derived from EGFP-injected embryos manipulated under 100 units/ml catalase (29%) was significantly higher (P<0.05) than that manipulated under 0 units/ml catalase (19%). Furthermore, the numbers of transgenic pups were 17 in 100 units/ml catalase and 14 in 0 units/ml catalase. The results of the present study indicate that scavenging reactive oxygen species during in vitro micromanipulation is beneficial for the development of DNA-injected embryos.
著者
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Ueda Masatsugu
The Ys Inst. Inc.
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HASHIMOTO Shu
The YS Institute Inc.
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KURAMOCHI Takashi
The YS Institute Inc.
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AOYAGI Kazuki
The YS Institute Inc.
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TAKAHASHI Ri-ichi
The YS Institute Inc.
関連論文
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- カタラーゼを添加したバッファー中でDNAを顕微注入すると胚の発育能力の低下を軽減できる
- Refined Porcine Follicle Stimulating Hormone Promotes the Responsiveness of Rabbits to Multiple-Ovulation Treatment
- Catalase in Manipulation Buffer Enhances the Developmental Competence of DNA-injected Embryos
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- Transgenic Animals with Various YAC Constructs : Preparation of 400 kb YAC DNA for Microinjection
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