Establishment of tsA58 Transgenic Rats as a Source of Conditionally Immortalized Cell Lines with Differentiated Functions
スポンサーリンク
概要
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To isolate a variety of rat cell lines with differentiated functions, we developed transgenic rat lines that ubiquitously express the temperature-sensitive large T-antigen gene of the simian virus 40 (SV40) tsA58 mutant under the control of the SV40 large T-antigen promoter. These rats might be advantageous for simultaneously establishing cell lines from different tissues of rats with the same genetic origin. The transgenic rat lines transmit a functional copy of the transgene and were bred with sib mating to generate the homozygous transgene. The established cell lines from this transgenic rat had temperature dependent growth and retained some of the differentiated functions of each particular tissue, and were useful as a ready source of novel conditionally immortalized cell lines. The possible use and perspectives of these transgenic cell lines are discussed.
- Japanese Association for Laboratory Animal Scienceの論文
- 2002-04-00
著者
関連論文
- Generation of Transgenic Rats with YACs and BACs: Preparation Procedures and Integrity of Microinjected DNA
- A comparative Study on the Integration of Exogenous DNA into Mouse, Rat, Rabbit, and Pig Genomes
- Development of a Conditionally Immortalized Testicular Sertoli Cell Line RTS3-3 from Adult Transgenic Rats Harboring Temperature-Sensitive Simian Virus 40 Large T-antigen Gene
- Development and Characterization of Conditionally Immortalized Gastric Epithelial Cell Lines from Transgenic Rats Harboring Temperature-Sensitive Simian Virus 40 Large T-antigen Gene
- Establishment of SV40-tsA58 Transgenic Rats as a Source of Conditionally Immortalized Cell Lines
- Viability of Transgenic Rat Embryos after Freezing and Thawing
- Transgenic Animals with Various YAC Constructs : Preparation of 400 kb YAC DNA for Microinjection
- O-5 Production of Transgenic Rats Showing Immunological tolerance to a Human Protein by the Introduction of a Fusion Gene Expressing under a Control of Milk Protein Gene Promoter.(Proceedings of the 27th Annual Meeting)
- Establishment of tsA58 Transgenic Rats as a Source of Conditionally Immortalized Cell Lines with Differentiated Functions