Production of Sei Whale (Balaenoptera borealis) Cloned Embryos by Inter- and Intra-Species Somatic Cell Nuclear Transfer
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概要
- 論文の詳細を見る
The objectives of this study were to choose an effective embryo reconstruction method and an effective post-activation agent for in vitro production of sei whale (Balaenoptera borealis) interspecies somatic cell nuclear transfer (iSCNT) embryos. Moreover, trichostatin A (TSA) treatment of whale iSCNT embryos was performed to improve the in vitro embryo development. In Experiment 1, the fusion rate was significantly higher (88.1%) in embryos reconstructed using the intracytoplasmic cell injection method (ICI) than that (48.7%) in the subzonal cell insertion (SUZI) counterpart. The rates of pseudopronucleus (PPN) formation (77.4 vs. 77.2%) and cleavage (24.5 vs. 37.0%) did not vary between ICI and SUZI. However, the PPN formation and cleavage rates were significantly (P<0.05) lower in the iSCNT embryos than in the parthenogenetic control (95.7% and 64.4%, respectively). Although 21.5% of the bovine parthenogenetic embryos developed to the blastocyst stage, no iSCNT embryo developed beyond the 6-cell stage. In Experiment 2, the cleavage rate did not vary between the TSA (50 nM)-treated and non-treated whale iSCNT embryos (30.5 vs. 32.3%, respectively). Moreover, it did not vary between the TSA-treated iSCNT and SCNT embryos (30.5 vs. 32.0%, respectively). Only one TSA non-treated iSCNT embryo developed to a compacted morula with 20 nuclei. One TSA-treated whale SCNT embryo developed to the 8-cell stage, and out of five whale iSCNT embryos, a 6-cell stage embryo was positive for whale DNA. In conclusion, bovine oocytes have the ability to support development of sei whale nuclei up to the 6-cell stage.
- 日本繁殖生物学会の論文
著者
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Fujise Yoshihiro
The Institute of Cetacean Research
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FUKUI Yutaka
Laboratory of Animal Reproduction, Obihiro University of Agriculture and Veterinary Medicine
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WATANABE Hiroyuki
Laboratory of Animal Reproduction, Obihiro University of Agriculture and Veterinary Medicine
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ISHIKAWA Hajime
The Institute of Cetacean Research
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OHSUMI Seiji
The Institute of Cetacean Research
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Suzuki Yo
Laboratory Of Animal Reproduction Obihiro University Of Agriculture And Veterinary Medicine
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Fukui Yutaka
Laboratory Of Animal Reproduction Obihiro University Of Agriculture And Veterinary Medicine
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Bhuiyan Mohammad
Laboratory Of Animal Reproduction Obihiro University Of Agriculture And Veterinary Medicine
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Watanabe H
Laboratory Of Animal Reproduction Obihiro University Of Agriculture And Veterinary Medicine
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Hirayama Hiroki
Reproductive Biotechnology Laboratory Hokkaido Animal Research Center
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LEE Eunsong
School of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University
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MATSUOKA Koji
The Institute of Cetacean Research
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Lee Eunsong
School Of Veterinary Medicine And Institute Of Veterinary Science Kangwon National University
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Watanabe Hiroyuki
Laboratory Of Animal Reproduction Obihiro University Of Agriculture And Veterinary Medicine
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Fukui Yutaka
Laboratory Of Animal Genefics And Reproduction Obihiro University Of Agriculture And Veterinary Medi
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OHSUMI Seiji
The Whales Research Institute
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