凍結融解ヒツジおよびミンククジラ精子における精子性状検査器と低張膨化試験の有効性
スポンサーリンク
概要
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The object of the present study was to investigate the validation of the sperm quality analyzer (SQA) and the hypo-osmotic swelling (HOS) test with standard sperm analysis methods in frozen-thawed ram and minke whale spermatozoa. In rams, highly significant correlations were observed in the percentage of motile spermatozoa (P<0.01) and sperm concentration (P<0.01) between the standard and SQA methods. But, the percentage of morphologically normal spermatozoa did not significantly correlate between the standard and SQA methods. The percentages of swollen spermatozoa at 15 minutes by the HOS test were significantly correlated with the motility by the standard (P<0.05) and by the SQA (P<0.05) methods. For minke whale spermatozoa, the SVI (sperm viability index) values by the standard method were significantly (P<0.001) correlated with the sperm motility index (SMI) values by SQA. The percentage of motile spermatozoa was also significantly correlated (P<0.01) with the motility measured by SQA. Using different hypo-osmotic solutions and incubation times, the HOS test with 25, 100 and 150 mOsM did not show significant variations. Motility observed by the standard method and the percentage of swollen spermatozoa were significantly correlated (P<0.05). These results indicate that the SQA and HOS test can be utilized to assess the post-thawing motility of ram and minke whale spermatozoa, and that the SQA and HOS test values are significantly correlated in ram spermatozoa. However, sperm concentration and morphologically normal spermatozoa are not assessed accurately by SQA in minke whales.
著者
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福井 豊
帯広畜産大学 畜産科学科・家畜増殖学研究室
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FUKUI Yutaka
Laboratory of Animal Reproduction, Obihiro University of Agriculture and Veterinary Medicine
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OHSUMI Seiji
The Institute of Cetacean Research
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Iida Kenji
Laboratory Of Biochemistry Department Of Agricultural Chemistry Kyoto University
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Ohsumi Seiji
Institute Of Cetacean Research
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Fukui Yutaka
Laboratory Of Animal Reproduction Obihiro University Of Agriculture And Veterinary Medicine
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福井 豊
帯広畜産大学家畜育種増殖学講座
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福井 豊
Laboratory of Meat Animal Reproduction, Obihiro University of Agriculture and Veterinary Medicine
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阿部 憲人
帯広畜産大学・畜産科学科・家畜増殖学研究室
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外川 守彦
Laboratory of Animal Reproduction, Department of Animal Production Science, Obihiro University of Ag
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阿部 憲人
Laboratory of Animal Reproduction, Department of Animal Production Science, Obihiro University of Ag
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TAKANO Yuuki
Laboratory of Animal Reproduction, Department of Animal Production Science, Obihiro University of Ag
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ASADA Masatsugu
Laboratory of Animal Reproduction, Department of Animal Production Science, Obihiro University of Ag
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OKADA Aki
Laboratory of Animal Reproduction, Department of Animal Production Science, Obihiro University of Ag
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ISHIKAWA Hajime
Institute of Cetacean Research
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TOGAWA Morihiko
Laboratory of Animal Genetics and Reproduction, Obihiro University of Agriculture and Veterinary Med
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ABE Norihito
Laboratory of Animal Reproduction, Department of Animal Production Science, Obihiro University of Ag
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外川 守彦
帯広畜産大学・畜産科学科・家畜増殖学研究室
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Okada Aki
Laboratory Of Animal Genefics And Reproduction Obihiro University Of Agriculture And Veterinary Medi
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Fukui Yutaka
Laboratory Of Animal Genefics And Reproduction Obihiro University Of Agriculture And Veterinary Medi
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OHSUMI Seiji
The Whales Research Institute
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ASADA Masatsugu
Laboratory of Animal Genetics and Reproduction, Obihiro University of Agriculture and Veterinary Medicine
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ASADA Masatsugu
Laboratory of Animal Reproduction, Department of Animal Production Science, Obihiro University of Agriculture and Veterinary Medicine
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Ishikawa Hajime
Institute for Solid State Physics, University of Tokyo, Kashiwa, Chiba 277-8581, Japan
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OKADA Aki
Laboratory of Animal Reproduction, Department of Animal Production Science, Obihiro University of Agriculture and Veterinary Medicine
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TOGAWA Morihiko
Laboratory of Animal Reproduction, Department of Animal Production Science, Obihiro University of Agriculture and Veterinary Medicine
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ABE Norihito
Laboratory of Animal Reproduction, Department of Animal Production Science, Obihiro University of Agriculture and Veterinary Medicine
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