ウシ黄体における黄体形成ホルモン(LH)のホスホリパーゼCを介したプロジェステロン分泌促進機構に関する研究
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概要
- 論文の詳細を見る
Luteinizing hormone (LH)-stimulated steroidogenesis in luteal cells is known to be mediated through the activation of cyclic AMP (cAMP)-dependent protein kinase, and to be also modulated by calcium-dependent mechanisms. In the present study, we tested the hypothesis that LH stimulates progesterone (P4) production in bovine luteal cells through activation of phospholipase (PL) C by using a cell culture system. Bovine mid-luteal cells (Days 8-12 of the estrous cycle) were cultured for 24 h and then exposed to a PLC inhibitor (U-73122; 10 μM) with or without LH (10 ng/ml) for 4 h. U-73122 blocked LH-stimulated P4 production without affecting cAMP accumulation. Moreover, exposure of luteal cells to PLC increased P4 production in a dose-dependent manner. These results support the hypothesis that the luteotropic action of LH in bovine luteal cells is mediated not only by activation of adenylate cyclase but also by activation of PLC.
- 日本繁殖生物学会の論文
著者
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OKUDA Kiyoshi
Laboratory of Reproductive Endocrinology, Okayama University
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SKARZYNSKI Dariusz
Department of Reproductive Immunology, Institute of Animal Reproduction and Food Research, Polish Ac
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柴谷 雅美
Laboratory of Reproductive Endocrinology, Faculty of Agriculture, Okayama University
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西村 亮
Laboratory of Reproductive Endocrinology, Graduate School of Natural Science and Technology, Okayama
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