Phyto- and Endogenous Estrogens Differently Activate Intracellular Calcium Ion Mobilization in Bovine Endometrial Cells
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概要
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The main purpose of this study was to check whether phyto- and endogenous estrogens influence calcium ion mobilization [Ca2+]i in bovine endometrial cells and whether this action is connected with biological effects i.e. prostaglandin (PG)F2α production. In our study we used two calcium measurement methods by comparing the microscopic method with widely used quantitative - spectrofluorometric method of [Ca2+]i measurement. We also wanted to confirm whether visualization of calcium ion [Ca2+] i in cells using microscopic method supported by micro image analysis (Micro Image Olympus system) reflects real, qualitative changes in the ion concentration. In both methods a cell-permeable form of fluorescent [Ca2+]i indicator Fura-2 was used. Cultured bovine endometrial epithelial and stromal cells influenced by phorbol-2-myristate-13-acetate (PMA; positive control), estradiol 17-β (E2; endogenous estrogen) and active metabolites of phytoestrogens (environmental estrogens) were used as a model to study PGF2α secretion and [Ca2+]i mobilization in the cells. Equol and para-ethyl-phenol in doses of 10-8-10-6 M increased PGF2α concentration both in epithelial and stromal cells (P<0.05). In both methods, equol and para-ethyl-phenol did not cause intracellular [Ca2+] i mobilization in epithelial and stromal cells (P>0.05). Both methods revealed that only E2 and PMA induced intracellular [Ca2+]i mobilization in epithelial and stromal cells (P<0.05). The results of both methods were highly correlated (P<0.001; r=0.82 for epithelial cells and r=0.89 for stromal cells). In conclusion, both methods gave approximately the same results and showed that phytoestrogens, in contrast to PMA and E2, did not cause intracellular [Ca2+] i mobilization in endometrial cells. The obtained results proved that the [Ca2+]i visualization method supported by micro image analysis can produce similar results to the spectrofluorometric method.
- 日本繁殖生物学会の論文
- 2006-12-01
著者
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OKUDA Kiyoshi
Laboratory of Reproductive Endocrinology, Okayama University
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Okuda Kiyoshi
Lab. Of Reproductive Endocrinology Graduate School Of Natural Sci. And Technol. Okayama Univ.
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Okuda Kiyoshi
Laboratory Of Reproductive Endocrinology Graduate School Of Natural Science And Technology Okayama U
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KORZEKWA Anna
Department of Reproductive Immunology, Institute of Animal Reproduction and Food Research, Polish Ac
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WOCLAWEK-POTOCKA Izabela
Department of Reproductive Immunology, Institute of Animal Reproduction and Food Research, Polish Ac
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SKARZYNSKI Dariusz
Department of Reproductive Immunology, Institute of Animal Reproduction and Food Research, Polish Ac
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奥田 潔
Laboratory Of Reproductive Endocrinology Graduate School Of Natural Science And Technology Okayama U
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BORKOWSKI Krzysztof
OLYMPUS Poland
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奥田 潔
岡山大学大学院自然科学研究科バイオサイエンス専攻生殖内分泌学
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Skarzynski Dariusz
Department Of Reproductive Immunology Institute Of Animal Reproduction And Food Research Polish Acad
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Okuda Kiyoshi
Okayama Univ. Okayama
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Korzekwa Anna
Department Of Reproductive Immunology Institute Of Animal Reproduction And Food Research Polish Acad
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Skarzynski Dariusz
Dep. Of Reproductive Immunology And Pathology Inst. Of Animal Reproduction And Food Res. Polish Acad
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Woclawek-potocka Izabela
Dep. Of Reproductive Immunology And Pathology Inst. Of Animal Reproduction And Food Res. Polish Acad
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Woclawek-potocka Izabela
Department Of Reproductive Immunology And Pathology Institute Of Animal Reproduction And Food Resear
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Woclawek-potocka Izabela
Department Of Reproductive Immunology Institute Of Animal Reproduction And Food Research Polish Acad
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Skarzynski Dariusz
Department Of Reproductive Immunology And Pathology Institute Of Animal Reproduction And Food Resear
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Skarzynski D
Department Of Reproductive Immunology Institute Of Animal Reproduction And Food Research Polish Academy Of Science
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