一酸化窒素(NO)はウシ黄体細胞のアポトーシスを誘導する
スポンサーリンク
概要
- 論文の詳細を見る
We previously showed in in vivo and in vitro studies that nitric oxide (NO) is engaged in luteolysis in cattle. Nitric oxide produced locally in the bovine corpus luteum (CL) inhibits progesterone (P4) synthesis and is suggested to be a component of the luteolytic cascade induced by uterine prostaglandin (PG) F2α. In the present study, the molecular mechanisms of NO action during structural luteolysis were studied in cultured bovine luteal cells (Days 15-17 of the estrous cycle). The effects of the NO donor (NONOate; 10-4M) on DNA fragmentation, cell viability, P4 production and caspase-3 activity were compared with those of PGF2α (10 -6M). Moreover, mobilization of intracellular calcium [Ca2+]i and gene expressions of Fas-L, Fas, bcl-2, bax, and caspase-3 in the cells were determined by semi-quantitative RT-PCR after NONOate treatment. Caspase-3 activity was examined calorimetrically. Contrary to PGF2α NONOate decreased cell viability. DNA fragmentation after NONOate treatment increased by more than with PGF2α. NONOate increased mobilization of [Ca2+]i in the cells. Although the NO donor did not affect Fas-L and bcl-2 gene expression, it stimulated Fas and bax mRNA and caspase-3 expression. The ratio of bcl-2 to bax mRNA level decreased in the cells treated with NONOate. Moreover, NONOate stimulated caspase-3 activity more effectively than PGF2α. The overall results suggest that NO is a luteolytic factor that plays a crucial role in regulation of the estrous cycle in structural luteolysis by inducing apoptosis of luteal cells in cattle.
- 日本繁殖生物学会の論文
- 2006-06-01
著者
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奥田 潔
岡山大学大学院自然科学研究科動物生殖内分泌学研究室
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Okuda Kiyoshi
Lab. Of Reproductive Endocrinology Graduate School Of Natural Sci. And Technol. Okayama Univ.
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KORZEKWA Anna
Department of Reproductive Immunology, Institute of Animal Reproduction and Food Research, Polish Ac
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WOCLAWEK-POTOCKA Izabela
Department of Reproductive Immunology, Institute of Animal Reproduction and Food Research, Polish Ac
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村上 周子
岡山大学大学院自然科学研究科バイオサイエンス専攻生殖内分泌学
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SKARZYNSKI Dariusz
Department of Reproductive Immunology, Institute of Animal Reproduction and Food Research, Polish Ac
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奥田 潔
Laboratory Of Reproductive Endocrinology Graduate School Of Natural Science And Technology Okayama U
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奥田 潔
岡山大学大学院自然科学研究科・生体制御学研究室
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村上 周子
岡山大学・農学部
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奥田 潔
岡山大学大学院自然科学研究科バイオサイエンス専攻生殖内分泌学
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Skarzynski Dariusz
Department Of Reproductive Immunology Institute Of Animal Reproduction And Food Research Polish Acad
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Okuda Kiyoshi
Okayama Univ. Okayama
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Korzekwa Anna
Department Of Reproductive Immunology Institute Of Animal Reproduction And Food Research Polish Acad
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Woclawek-potocka Izabela
Dep. Of Reproductive Immunology And Pathology Inst. Of Animal Reproduction And Food Res. Polish Acad
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Woclawek-potocka Izabela
Department Of Reproductive Immunology And Pathology Institute Of Animal Reproduction And Food Resear
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Skarzynski Dariusz
Department Of Reproductive Immunology And Pathology Institute Of Animal Reproduction And Food Resear
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Korzekwa Anna
Department Of Reproductive Immunology Institute Of Animal Reproduction And Food Research Polish Academy Of Sciences
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