Development and Evaluation of a Rapid Enzyme-immunoassay System for Measurement of the Urinary Concentration of Estrone-3-glucuronide in a Female Giant Panda (Ailuropoda melanoleuca)
スポンサーリンク
概要
- 論文の詳細を見る
To detect estrus for reproductive management, and to determine the relationship between urinary estrogen and estrous behavior, in a female giant panda, we developed and evaluated a rapid enzyme immunoassay (EIA) system for urinary Estrone-3-gluculonide (E1G) using commercial reagents. The developed EIA system took only around 3 hours, including all procedures to obtain a result. It indicated good reproducibility (intra-assay CV of 5.16%, interassay CV of 15.4%) and sensitivity (lowest standard concentration was 0.0156 ng/ml) for measurement of the urinary concentrations of E1G in the giant panda. There was a positive correlation (r=0.934) with the data for estrone (E1) in the same samples, as measured by radioimmunoassay (RIA) performed in a commercial laboratory. The changes in the E1G concentrations were almost synchronous with the changes in E1 assayed by RIA in urine collected during 4 consecutive estrous seasons. The dynamics of urinary E1G measured by this system highly correlated with the occurrence of the presenting estrous behavior in the giant panda. The above results indicate that this assay system may be normally, rapidly and practically used for measurement of the urinary concentration of E1G in the giant panda.
- 日本繁殖生物学会の論文
- 2008-08-01
著者
-
Hama Natsuki
Graduate School of Natural Science and Technology, Okayama University
-
Kanemitsu Hideyasu
Kobe Municipal Oji Zoo
-
SAKAMOTO Kensuke
Kobe Municipal Oji Zoo
-
OYAMA Yujiro
Kobe Municipal Oji Zoo
-
ACOSTA Tomas
Graduate School of Natural Science and Technology, Okayama University
-
ISHIKAWA Osamu
Kobe Municipal Oji Zoo
-
OKUDA Kiyoshi
Graduate School of Natural Science and Technology, Okayama University
-
Okuda Kiyoshi
Lab. Of Reproductive Endocrinology Graduate School Of Natural Sci. And Technol. Okayama Univ.
-
Acosta Tomas
Lab. Of Reproductive Endocrinology Graduate School Of Natural Sci. And Technol. Okayama Univ.
-
奥田 潔
Laboratory Of Reproductive Endocrinology Graduate School Of Natural Science And Technology Okayama U
-
Oyama Yujiro
Kobe Municipal Oji Zoo Kobe Jpn
-
Pengyan Wang
China Res. And Conservation Center For The Giant Panda Sichuan Chn
-
Acosta Tomas
Graduate School Of Natural Science And Technology Okayama University
-
Sakamoto Kensuke
Kobe Municipal Oji Zoo Kobe Jpn
-
奥田 潔
岡山大学大学院自然科学研究科バイオサイエンス専攻生殖内分泌学
-
Okuda Kiyoshi
Graduate School Of Natural Science And Technology Okayama University
-
Ishikawa Osamu
Kobe Municipal Oji Zoo Kobe Jpn
-
Okuda Kiyoshi
Okayama Univ. Okayama
-
WANG PENGYAN
China Research and Conservation Center for the Giant Panda
-
Okuda Kiyoshi
Graduate School Of Natural Sci. And Technol. Okayama Univ.
-
ACOSTA Tomas
Graduate School of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine
関連論文
- ジャイアントパンダ(Ailuropoda melanoleuca)における人工授精と死産
- Expression of VEGF and Its Receptors in the Bovine Endometrium Throughout the Estrous Cycle : Effects of VEGF on Prostaglandin Production in Endometrial Cells
- Development of an Enzyme Immunoassay for Urinary Pregnanediol-3-Glucuronide in a Female Giant Panda (Ailuropoda melanoleuca)
- 牛初期胚発育に及ぼすインターフェロンτの作用
- 一酸化窒素(NO)はウシ黄体細胞のアポトーシスを誘導する
- 日本繁殖生物学会賞・奨励賞 卵巣機能調節機構における腫瘍壊死因子の生理的役割
- ウシ顆粒層細胞および卵胞膜内膜細胞における腫瘍壊死因子(TNFα)受容体
- 正常発情周期中のブタ子宮内膜ではトロフィニンが発現している
- ウシ生体を用いた黄体形成期における黄体内のプロスタグランジン(PG)F2αおよびPGE2濃度の経時的変化
- 雌仔ウシおよび成熟雌ウシの末梢血中プロジェステロンとエンドセリン濃度の変化 : PGF_投与の影響
- 黄体と子宮の機能調節におけるアポトーシスの役割とその制御
- Species-Related Differences in the Mechanism of Apoptosis During Structural Luteolysis
- Autographa californica nuclear polyhedrosis virus 発現系により作出した組換えウシインターフェロンτの生物活性
- Luteotropic Mechanisms in the Bovine Corpus Luteum : Role of Oxytocin, Prostaglandin F_, Progesterone and Noradrenaline
- Acute Changes in Circulating Concentrations of Progesterone and Nitric Oxide and Partial Pressure of Oxygen During Prostaglandin F_-induced Luteolysis in Cattle
- Changes in the Vasculature of Bovine Corpus Luteum During the Estrous Cycle and Prostaglandin F2α-induced Luteolysis
- Possible Roles of Intracellular Cyclic AMP, Protein Kinase C and Calcium Ion in the Apoptotic Signaling Pathway in Bovine Luteal Cells
- Prostaglandin F_ Regulates the Nitric Oxide Generating System in Bovine Luteal Endothelial Cells
- ホルスタイン種雄牛の加齢にともなう血中ホルモン濃度の変化
- Nitric oxide in bovine corpus luteum : Possible mechanisms of action in luteolysis
- Effective Oxytocin Treatment on Placental Expulsion after Foaling in Heavy Draft Mares(Theriogenology)
- Relationship between Peripartal Plasma Oxytocin and Prostaglandin F_ Metabolite and Placental Expulsion Time in Heavy Draft Mares
- A Potential Use of Color Ultrasound as a Tool for Reproductive Management : New Observations Using Color Ultrasound Scanning that were not Possible with Imaging Only in Black and White
- Phyto- and Endogenous Estrogens Differently Activate Intracellular Calcium Ion Mobilization in Bovine Endometrial Cells
- Leydig Cell Hyperplasia in an ENU-induced Mutant Mouse with Germ Cell Depletion
- ウシにおいて排卵前の卵胞内でコルチゾールの局所産生が増加する生体内での証拠
- Leydig Cell Hyperplasia in an ENU-induced Mutant Mouse with Germ Cell Depletion
- Development and Evaluation of a Rapid Enzyme-immunoassay System for Measurement of the Urinary Concentration of Estrone-3-glucuronide in a Female Giant Panda (Ailuropoda melanoleuca)
- Effects of Prostaglandin F_ and Nitric Oxide on the Secretory Function of Bovine Luteal Cells
- Effects of Nitric Oxide and Tumor Necrosis Factor-α on Production of Prostaglandin F_ and E_2 in Bovine Endometrial Cells
- Progesterone Stimulation by LH Involves the Phospholipase-C Pathway in Bovine Luteal Cells
- Progesterone Stimulation by Prostaglandin F2α Involves the Protein Kinase C Pathway in Cultured Bovine Luteal Cells
- Age-Related Changes in the Serum Levels of Inhibin, FSH, LH and Testosterone in Holstein Bulls
- HEPATOCYTE GROWTH FACTOR AS A LOCAL REGULATOR IN BOVINE ENDOMETRIUM
- Three-dimensional analysis of the manipulation system in the lesser panda
- PREGNANCY DIAGNOSIS BY ULTRASONIC SCANNING AND MEASUREMENT OF SERUM PROGESTERONE LEVELS IN EZO SIKA DOES (Cervus nippon yesoensis HEUDE)
- In Vivo Evidence that Local Cortisol Production Increases in the Preovulatory Follicle of the Cow
- 卵巣機能調節機構における腫瘍壊死因子の生理的役割
- ウシ顆粒層細胞および卵胞膜内膜細胞における腫瘍壊死因子(TNFα)受容体
- ウシ成熟卵胞を微透析した灌流液中におけるGnRH-immunoreactivityの存在
- Changes in Follicular Vascularity during the First Follicular Wave in Lactating Cows
- 発情周期中およびプロスタグランジンF2α投与後のウシ黄体内でのアンジオポエチンと受容体TieのmRNA発現の変化:黄体退行開始機構への関与の可能性
- Effects of Tumor Necrosis Factor α and Interferon γ on the Viability and mRNA Expression of TNF Receptor Type I in Endothelial Cells from the Bovine Corpus Luteum
- Comparative Activities of Growth Hormone and Luteinizing Hormone in the Direct Stimulation of Local Release of Progesterone from Microdialyzed Ovine Corpora Lutea In Vivo
- 不妊発情周期における周期回帰のメカニズム
- Progesterone Stimulation by Prostaglandin F_ Involves the Protein Kinase C Pathway in Cultured Bovine Luteal Cells
- Secretion of Prostaglandins E_2 and F_ by the Bovine Endometrium in Response to Oxytocin during the Estrous Cycle
- Epidermal Growth Factor and Its Receptor in Bovine Corpus Luteum during the Estrous Cycle
- Gene Expression of 11β-HSD and Glucocorticoid Receptor in the Bovine (Bos taurus) Follicle During Follicular Maturation and Atresia : The Role of Follicular Stimulating Hormone
- Effects of Cortisol on Pregnancy Rate and Corpus Luteum Function in Heifers : An In Vivo Study
- Relationship between Embryo Collection Results after Superovulation Treatment of Japanese Black Cows and Their Plasma β-carotene and Vitamin Concentrations
- Effect of the Dominant Follicle Aspiration before or after Luteinizing Hormone Surge on the Corpus Luteum Formation in the Cow